Integrin-dependent human macrophage migration induced by oscillatory electrical stimulation
Integrin-dependent human macrophage migration induced by oscillatory electrical stimulation
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DOI：
10.1114/1.263
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发表时间：
2000-03-01
影响因子：
3.8
通讯作者：
Golan, DE
中科院分区：
文献类型：
--
作者：
Cho, MR;Thatte, HS;Golan, DE
电刺激已被用于促进伤口愈合。这种刺激可以与生物系统相互作用以加速愈合的机制尚未阐明。一个潜在的机制可能涉及刺激巨噬细胞迁移到伤口部位。在这里，我们报告，振荡电场诱导人类巨噬细胞迁移。暴露于1 Hz、2 V/cm场的大分子在玻璃基片上显示出5.2+/-0.4 × 10（-2）μ m/min的诱导迁移速度和4.8+/-1.4 × 10（-2）μ m（2）/min的随机迁移系数。电场暴露诱导微丝从细胞周边的环状结构重组为被限制在细胞和基底之间的接触位点的足状体，这表明细胞在玻璃上爬行。在现场曝光之前，用针对Pz-整联蛋白的单克隆抗体处理细胞可以防止细胞迁移，这表明涉及整联蛋白依赖性信号传导途径。电场引起巨噬细胞在层粘连蛋白或纤连蛋白包被的基底上迁移，而不诱导足体形成或细胞形态变化。迁移速度没有显着改变，但随机运动被抑制，这表明层粘连蛋白或纤连蛋白包被的表面上的细胞运动不介导的细胞爬行。有人建议，电场诱导的巨噬细胞迁移利用几种模式的细胞运动，包括细胞爬行和可能的细胞滚动。(C)2000生物医学工程学会。【S0090-6964（00）00903-6】。
Electrical stimulation has been used to promote wound healing. The mechanisms by which such stimulation could interact with biological systems to accelerate healing have not been elucidated. One potential mechanism could involve stimulation of macrophage migration to the site of a wound. Here we report that oscillatory electric fields induce human macrophage migration. Macrophages exposed to a 1 Hz, 2 V/cm field show an induced migration velocity of 5.2+/-0.4 ii 10(-2) mu m/min and a random motility coefficient of 4.8+/- 1.4 X 10(-2) mu m(2)/min on a glass substrate. Electric field exposure induces reorganization of microfilaments from ring-like structures at the cell periphery to podosomes that are confined to the contact sites between cell and substrate, suggesting that the cells are crawling on glass. Treatment of cells with monoclonal antibodies directed against Pz-integrins prior to field exposure prevents cell migration, indicating that integrin-dependent signaling pathways are involved. Electric fields cause macrophage migration on laminin or fibronectin coated substrates without inducing podosome formation or changes in cellular morphology. The migration velocity is not significantly altered but the random movement is suppressed, suggesting that cell movements on a laminin- or fibronectin-coated surface are not mediated by cell crawling. It is suggested that electric field-induced macrophage migration utilizes several modes of cell movement, including cell crawling and possibly cell rolling. (C) 2000 Biomedical Engineering Society. [S0090-6964(00)00903-6].