A phylogeographical analysis of the Bemisia tabaci species complex based on mitochondrial DNA markers
A phylogeographical analysis of the Bemisia tabaci species complex based on mitochondrial DNA markers
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DOI：
10.1046/j.1365-294x.1999.00754.x
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发表时间：
1999-10-01
影响因子：
4.9
通讯作者：
Brown, JK
中科院分区：
文献类型：
--
作者：
Frohlich, DR;Torres-Jerez, I;Brown, JK
利用线粒体16 S（550 bp）和细胞色素氧化酶I（COI）（700 bp）序列作为分子标记，对烟粉虱代表性种群或生物型进行了生态地理重建。16 S序列比COI序列表现出更小的分歧。在COI序列检测的429个字符中，185个是不变的，244个是可变的，108个是信息性的。COI序列同一性产生的距离范围从小于1%到大于17%。对烟粉虱16 S序列的456个性状进行了分析，其中298个为不变位点，158个为可变位点，53个为信息位点。系统发育分析进行了最大简约，最大似然和邻居加入的方法产生了几乎相同的系统发育重建的树木，分离粉虱的地理起源的基础上。16 S和COI序列数据表明，B-生物型起源于旧世界（欧洲，亚洲和非洲），与以色列和也门的B-样变体关系最密切，下一个最近的亲戚是苏丹的生物型。这些数据证实了先前描述的B的生化、遗传和行为多态性。烟粉虱考虑了B的所有全局变量。烟粉虱是一个高度神秘的兄弟物种。
Mitochondrial 16S (similar to 550 bp) and cytochrome oxidase I (COI) (similar to 700 bp) sequences were utilized as markers to reconstruct a phylogeography for representative populations or biotypes of Bemisia tabaci. 16S sequences exhibited less divergence than COI sequences. Of the 429 characters examined for COI sequences, 185 sites were invariant, 244 were variable and 108 were informative. COI sequence identities yielded distances ranging from less than 1% to greater than 17%. Whitefly 16S sequences of 456 characters were analysed which consisted of 298 invariant sites, 158 variable sites and 53 informative sites. Phylogenetic analyses conducted by maximum parsimony, maximum-likelihood and neighbour-joining methods yielded almost identical phylogenetic reconstructions of trees that separated whiteflies based on geographical origin. The 16S and COI sequence data indicate that the B-biotype originated in the Old World (Europe, Asia and Africa) and is most closely related to B-like variants from Israel and Yemen, with the next closest relative being a biotype from Sudan. These data confirm the biochemical, genetic and behavioural polymorphisms described previously for B. tabaci. The consideration of all global variants of B. tabaci as a highly cryptic group of sibling species is argued.