Engineered Sorghum Bagasse Enables a Sustainable Biorefinery with p ‐Hydroxybenzoic Acid‐Based Deep Eutectic Solvent
Engineered Sorghum Bagasse Enables a Sustainable Biorefinery with p ‐Hydroxybenzoic Acid‐Based Deep Eutectic Solvent
复制标题
工程化高粱甘蔗渣利用对羟基苯甲酸基低共熔溶剂实现可持续生物精炼
DOI：
10.1002/cssc.202101492
复制
发表时间：
2021
期刊：
影响因子：
8.4
通讯作者：
Ragauskas, Arthur J.
中科院分区：
文献类型：
--
作者：
Wang, Yunxuan;Meng, Xianzhi;Tian, Yang;Kim, Kwang Ho;Jia, Linjing;Pu, Yunqiao;Leem, Gyu;Kumar, Deepak;Eudes, Aymerick;Ragauskas, Arthur J.
整合植物基因工程和可再生低共熔溶剂（DES）的多学科研究可以促进可持续和经济的生物精炼。在此，我们利用植物基因工程方法将C6 C1单体特异性地掺入木质素结构中。通过在高粱中表达细菌alubiC基因，富含对羟基苯甲酸（PB）的木质素被整合到植物细胞壁中，而野生型（WT）生物质的木质素中完全不存在该单体。用氯化胆碱（ChCl）和PB合成DES，并将其应用于富含PB的突变生物质的预处理，用于可持续的生物精炼。通过DES预处理，与未处理的生物质相比，可发酵糖的释放显著增强（增加约190%）。特别地，从预处理的突变生物质释放的葡萄糖比从预处理的WT生物质释放的葡萄糖高多达12%。木质素被有效地从生物质中去除，保留了超过一半的β-0 - 4键而没有稠合的芳族结构。对分级木质素进行氢解以证明酚类化合物生产的潜力。此外，简单的水热处理可以从相同的工程木质素中选择性地提取PB，显示出可能的循环生物炼制。这些结果表明，基于PB的DES和工程化的富含PB的生物质的组合是实现可持续闭环生物精炼的有前途的策略。
Integrating multidisciplinary research in plant genetic engineering and renewable deep eutectic solvents (DESs) can facilitate a sustainable and economic biorefinery. Herein, we leveraged a plant genetic engineering approach to specifically incorporate C6C1monomers into the lignin structure. By expressing the bacterialubiCgene in sorghum,p‐hydroxybenzoic acid (PB)‐rich lignin was incorporated into the plant cell wall while this monomer was completely absent in the lignin of the wild‐type (WT) biomass. A DES was synthesized with choline chloride (ChCl) and PB and applied to the pretreatment of the PB‐rich mutant biomass for a sustainable biorefinery. The release of fermentable sugars was significantly enhanced (∼190 % increase) compared to untreated biomass by the DES pretreatment. In particular, the glucose released from the pretreated mutant biomass was up to 12 % higher than that from the pretreated WT biomass. Lignin was effectively removed from the biomass with the preservation of more than half of the β‐Ο‐4 linkages without condensed aromatic structures. Hydrogenolysis of the fractionated lignin was conducted to demonstrate the potential of phenolic compound production. In addition, a simple hydrothermal treatment could selectively extract PB from the same engineered lignin, showing a possible circular biorefinery. These results suggest that the combination of PB‐based DES and engineered PB‐rich biomass is a promising strategy to achieve a sustainable closed‐loop biorefinery.