A QTL detected in an interspecific pear population confers stable fire blight resistance across different environments and genetic backgrounds
A QTL detected in an interspecific pear population confers stable fire blight resistance across different environments and genetic backgrounds
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DOI：
10.1007/s11032-016-0473-z
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发表时间：
2016-04
影响因子：
3.1
通讯作者：
S. Montanari;L. Perchepied;Déborah Renault;L. Frijters;R. Velasco;M. Horner;S. Gardiner;D. Chagné;V. Bus;C. Durel;M. Malnoy
中科院分区：
文献类型：
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作者：
S. Montanari;L. Perchepied;Déborah Renault;L. Frijters;R. Velasco;M. Horner;S. Gardiner;D. Chagné;V. Bus;C. Durel;M. Malnoy
由梨火疫病菌（Erwinia amylovora（Burrill）温斯洛et al.）是梨的主要病害之一。培育具有持久抗性的梨品种对于有效控制火疫病极为重要，也是世界上大多数梨育种计划的关键目标。对梨种间群体PEAR 3（PremP 003，P ×bretschneideri×P. communis）× 'Moonglow'（P. communis）在法国和新西兰两个不同地理位置的抗火疫病性。淀粉分解菌利用单核苷酸多态性（SNP）和微卫星（SSR）标记构建的遗传图谱，我们在“月光”的第2连锁群（LG）上检测到一个主效数量性状位点（QTL）（R2= 12.9- 34.4%），该位点在两种环境下都是稳定的。我们证明了该QTL与之前在“哈罗甜”中检测到的另一个抗火疫病主要QTL共定位，并且这两个有利的（即，抗性）等位基因在血统上不相同。我们还从感病亲本PEAR 3中鉴定了一些效应较小（R2= 8.1- 14.8%）的QTL。我们提出了与LG 2的大效应QTL连锁的SNP和SSR标记作为梨抗火疫病分子标记辅助育种的候选标记。
Fire blight, caused by the bacteriumErwinia amylovora(Burrill) Winslow et al., is one of the most serious diseases of pear. The development of pear cultivars with a durable resistance is extremely important for effective control of fire blight and is a key objective of most pear breeding programs throughout the world. We phenotyped seedlings from the interspecific pear population PEAR3 (PremP003,P.×bretschneideri×P. communis) × ‘Moonglow’ (P. communis) for fire blight resistance at two different geographic locations, in France and New Zealand, respectively, employing two localE. amylovoraisolates. Using a genetic map constructed with single nucleotide polymorphism (SNP) and microsatellite (SSR) markers previously developed for this segregating population, we detected a major quantitative trait locus (QTL) on linkage group (LG)2 of ‘Moonglow’ (R2= 12.9–34.4 %), which was stable in both environments. We demonstrated that this QTL co-localizes with another major QTL for fire blight resistance previously detected in ‘Harrow Sweet’ and that the two favorable (i.e., resistant) alleles were not identical by descent. We also identified some smaller effect (R2= 8.1–14.8 %) QTLs derived from the susceptible parent PEAR3. We propose SNP and SSR markers linked to the large effect QTL on LG2 as candidates for marker-assisted breeding for fire blight resistance in pear.