Biosynthesis of dicarboxylic acids by carbon dioxide fixation. II. Further study of the properties of the "malic" enzyme of pigeon liver.
Biosynthesis of dicarboxylic acids by carbon dioxide fixation. II. Further study of the properties of the "malic" enzyme of pigeon liver.
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DOI：
10.1016/s0021-9258(19)52329-8
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发表时间：
1950-12
期刊：
影响因子：
--
通讯作者：
J. B. VEIGA SALLES;S. Ochoa
中科院分区：
文献类型：
--
作者：
J. B. VEIGA SALLES;S. Ochoa
(Mn++）\n-苹果酸盐+TPN+随后进行免疫计量。由于乳酸脱氢酶的干扰，分光光度法不能用于测定反应1的平衡常数，乳酸脱氢酶污染了现有的最佳鸽肝酶制剂（1）。然而，通过与异柠檬酸脱氢酶系统偶联（反应2），得到反应3（3）。该体系的平衡有利于（2）α-酮戊二酸+COZ+ TPNH，
(Mn++)\n-malate’+ TPN+ followed spectrophotometrically. The spectrophotometric method could not be applied to the determination of the equilibrium constant of Reaction 1 owing to interference by lactic dehydrogenase which contaminated the best preparations of the pigeon liver enzyme available (1). However, through coupling with the isocitric dehydrogenase system (Reaction 2), Reaction 3 was obtained (3). The equilibrium of this system favored the (2) a-Ketoglutarate’+ COZ+ TPNH,