Facile formation of giant elastin-like polypeptide vesicles as synthetic cells

Facile formation of giant elastin-like polypeptide vesicles as synthetic cells
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作为合成细胞轻松形成巨型弹性蛋白样多肽囊泡

DOI:
10.1039/d1cc05579h
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发表时间:
2021
影响因子:
4.9
通讯作者:
Liu, Allen P
Liu, Allen P
中科院分区:
化学2区
文献类型:
--
作者:
Sharma, Bineet;Ma, Yutao;Hiraki, Harrison L;Baker, Brendon M;Ferguson, Andrew L;Liu, Allen P

文献摘要

相似文献

我们证明了通过使用乳液转移方法的简便和强大的生成的巨肽囊泡。这些强大的囊泡可以承受化学和物理压力。肽囊泡可以通过包封必需成分来宿主无细胞表达反应。我们展示了另一种无细胞表达的弹性蛋白样多肽掺入到肽囊泡的现有膜中。
We demonstrate the facile and robust generation of giant peptide vesicles by using an emulsion transfer method. These robust vesicles can sustain chemical and physical stresses. The peptide vesicles can host cell-free expression reactions by encapsulating essential ingredients. We show the incorporation of another cell-free expressed elastin-like polypeptide into the existing membrane of the peptide vesicles.