Neutrophil antiserum response to decrease in proteolytic activity in loaded rat muscle
Neutrophil antiserum response to decrease in proteolytic activity in loaded rat muscle
复制标题
中性粒细胞抗血清对负载大鼠肌肉中蛋白水解活性降低的反应
DOI：
10.1007/s004210170004
复制
发表时间：
2001
影响因子：
3
通讯作者：
V. Rogozkin
中科院分区：
文献类型：
--
作者：
V. Morozov;T. N. Usenko;V. Rogozkin
在激烈的肌肉活动后，在骨骼肌中发现的白细胞已被证明渗透到骨骼肌组织的损伤区域。我们认为，在白细胞脱颗粒或破坏后出现在组织中的白细胞酶可能在组织酶活性中发挥作用。检测中性粒细胞蛋白水解酶活性。组织学数据提供了大鼠肌肉组织在剧烈运动负荷后被白细胞渗透的证据。为减少白细胞进入大鼠肌肉，肌肉负荷后向大鼠注射兔抗大鼠腹膜白细胞抗血清。与对照组动物(接受生理盐水注射)相比，这导致肌肉胞浆蛋白分解活性降低。经抗血清处理的大鼠比目鱼肌条件培养液的蛋白分解活性也降低。这些数据提供了证据，证明在运动负荷后观察到的大鼠骨骼肌蛋白水解酶活性的增加部分是由中性粒细胞蛋白水解酶诱导的。
The leukocytes that are found in skeletal muscles after intense muscular activity have been shown to infiltrate areas of injury in skeletal muscle tissue. We believe that leukocyte enzymes, which appear in the tissue after the degranulation or destruction of leukocytes, could play a role in tissue enzyme activities. Neutrophil proteinases were investigated. Histological data provided evidence of rat muscular tissue infiltration by leukocytes after intense physical loading. To reduce the influx of leukocytes in rat muscles, a rabbit antiserum against rat peritoneal leukocytes was injected into rats after muscle loading. This resulted in a reduction in muscle cytosol proteolytic activity as compared to control animals (who received saline injections). The levels of proteolytic activities of media conditioned by the soleus muscle isolated from antiserum-treated rats were also reduced. These data provide evidence that the increase in proteolytic activity observed in rat skeletal muscles after physical loading is partially induced by neutrophil proteinases.