Effects of gut microbiota on the microRNA and mRNA expression in the hippocampus of mice
Effects of gut microbiota on the microRNA and mRNA expression in the hippocampus of mice
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肠道菌群对小鼠海马 microRNA 和 mRNA 表达的影响
DOI：
10.1016/j.bbr.2017.01.021
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发表时间：
2017-03-30
影响因子：
2.7
通讯作者：
Xie, Peng
中科院分区：
文献类型：
--
作者：
Chen, Jian-jun;Zeng, Ben-hua;Xie, Peng
背景资料：肠道菌群是一个重要的环境因子，通过菌群-肠道-脑轴影响脑功能和行为。方法：在这里，我们使用无菌（GF）小鼠来探讨肠道微生物群对海马微小RNA（miRNA）和信使RNA（mRNA）表达的影响。结果：行为测试表明，与无特定病原体（SPF）小鼠相比，GF小鼠表现出更多的中心时间和中心距离，对熟悉食物的潜伏期更短。用SPF小鼠的肠道微生物群定殖GF小鼠并没有逆转这些行为。而7个差异表达的miRNAs和139个mRNAs明显恢复。通过microRNA Target Filter分析，7个修复的miRNAs中有4个含有2232个靶mRNA。在这些靶mRNA中，21种靶mRNA水平降低。进一步分析表明，GO项中最显著的是代谢过程（GO：0008152），结合（GO：0005488）和细胞部分（GO：0044464）分别对生物学过程、分子功能和细胞组分的作用，并且最显著改变的途径是轴突引导结论：这些发现表明肠道微生物群对青春期GF小鼠的定殖不足以逆转行为改变。肠道菌群可显著影响海马组织中miRNAs和mRNAs的表达水平。本研究结果可为进一步研究微生物群-肠-脑轴提供有价值的原始数据。(C)2017爱思唯尔B. V.保留所有权利。
Backgrounds: Gut microbiota is increasingly recognized as an important environmental factor that could influence the brain function and behaviors through the microbiota-gut-brain axis. Method: Here, we used the germ-free (GF) mice to explore the effect of gut microbiota on hippocampal microRNA (miRNA) and messenger RNAs (mRNAs) expression.Results: Behavioral tests showed that, compared to specific pathogen-free (SPF) mice, the GF mice displayed more center time, center distance and less latency to familiar food. Colonization of the GF mice with gut microbiota from SPF mice did not reverse these behaviors. However, 7 differentially expressed miRNAs and 139 mRNAs were significantly restored. Through microRNA Target Filter analysis, 4 of 7 restored miRNAs had 2232 target mRNAs. Among these target mRNAs, 21 target mRNAs levels were decreased. Further analysis showed that the most significant GO terms were metabolic process (GO: 0008152), binding (GO: 0005488) and cell part (GO: 0044464) for biological process, molecular function and cellular component, respectively, and the most significantly altered pathway was axon guidance (mmu04360).Conclusions: These findings indicated that colonization of gut microbiota to adolescent GF mice was not sufficient to reverse the behavioral alterations. Gut microbiota could significantly influence the expression levels of miRNAs and mRNAs in hippocampus. Our results could provide original and valuable data for researchers to further study the microbiota-gut-brain axis. (C) 2017 Elsevier B.V. All rights reserved.