Influence of the amino acid composition on the ionization efficiencies of small peptides
Influence of the amino acid composition on the ionization efficiencies of small peptides
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DOI：
10.1002/jms.4348
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发表时间：
2019-06-01
影响因子：
2.3
通讯作者：
Kruve, Anneli
中科院分区：
文献类型：
--
作者：
Liigand, Piia;Kaupmees, Karl;Kruve, Anneli
电喷雾电离被广泛用于从小离子到大蛋白质的各种分子产生气相离子，而电离机制据称取决于分子的大小。对于小分子，电离效率，即电喷雾过程中产生的气相离子的量，取决于化合物的性质。在这里，我们表明，氨基酸组成也影响的寡肽的电离效率。此外，我们表明，由超过5个氨基酸残基组成的寡肽的电离效率是非常相似的，彼此，假设相等的电离效率是可行的。因此，准确的电离效率预测主要是小寡肽。对于这些寡肽，氨基酸组成可以用于以易于使用的方式估计电离效率。
Electrospray ionization is widely used to generate gas phase ions from a variety of molecules ranging from small ions to large proteins, while the ionization mechanism is claimed to depend on the size of the molecule. For small molecules, the ionization efficiency, the amount of gas phase ions produced in the electrospray process, depends on the properties of the compound. Here, we show that the amino acid composition also influences the ionization efficiency of the oligopeptide. Additionally, we show that the ionization efficiencies of oligopeptides consisting of more than five amino acid residues are very similar to one another, and assuming equal ionization efficiencies is feasible. Therefore, accurate ionization efficiency predictions are needed mainly for small oligopeptides. For these oligopeptides, the amino acid composition can be used to estimate the ionization efficiency in an easy to use manner.