INTERACTION BETWEEN LYMPHOCYTES AND CULTURED HIGH ENDOTHELIAL-CELLS - AN INVITRO MODEL OF LYMPHOCYTE MIGRATION ACROSS HIGH ENDOTHELIAL VENULE ENDOTHELIUM
INTERACTION BETWEEN LYMPHOCYTES AND CULTURED HIGH ENDOTHELIAL-CELLS - AN INVITRO MODEL OF LYMPHOCYTE MIGRATION ACROSS HIGH ENDOTHELIAL VENULE ENDOTHELIUM
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DOI：
10.1002/eji.1830180818
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发表时间：
1988-08-01
影响因子：
5.4
通讯作者：
MISTRY, S
中科院分区：
文献类型：
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作者：
AGER, A;MISTRY, S
应用光镜和电镜观察了淋巴细胞与培养的高内皮微静脉内皮细胞之间的相互作用。单层培养的高内皮细胞（HEC）与淋巴细胞的结合效率比主动脉内皮、主动脉成纤维细胞或血清包被玻璃高15-200倍。与HEC结合的淋巴细胞的光学显微镜显示两个群体。I型淋巴细胞为相亮、圆形，未显示细胞内细节。II型淋巴细胞为暗相和扁平。在高倍光镜下，II型细胞的细胞核和细胞质清晰可见。通过电子显微镜测定了这两个群体的相对位置。I型淋巴细胞附着在HEC表面，II型淋巴细胞在HEC下面变平。从I型到II型的转变伴随着表面微绒毛的损失和细胞内细胞器的重新分布。这表明在该测定中淋巴细胞主动迁移穿过HEC层。通过表面表型分析确定迁移的淋巴细胞与总粘附细胞的关系。淋巴细胞不会从接种的细胞群中随机粘附。60分钟后，B相对于T淋巴细胞富集，并且粘附T细胞群本身相对于CD 4+细胞富集CD 8+细胞。然而，迁移的细胞是粘附于HEC的淋巴细胞的随机亚群。这是迁移之前与HEC特异性结合的明确证据。淋巴细胞粘附是独立的活HEC表明，这是一个被动的事件的内皮的一部分。然而，淋巴细胞迁移完全依赖于存活的高内皮细胞。我们的结论是，培养的HEC提供了一种生物学相关的三维基质，支持淋巴细胞的特异性粘附并积极促进淋巴细胞迁移。这些观察结果提示，培养的高内皮细胞为研究淋巴细胞从血液迁移到淋巴结提供了一种新的体外模型。
The interaction between lymphocytes and cultured high endothelial venule endothelium has been studied using light and electron microscopy. High endothelial cells (HEC) in monolayer culture bound lymphocytes 15-200-fold more efficiently than aortic endothelium, aortic fibroblasts or serum-coated glass. Light microscopy of lymphocytes bound to HEC showed two populations. Type I lymphocytes were phase-light, round and revealed no intracellular detail. Type II lymphocytes were phase-dark and flattened. The nucleus and cytoplasm of type II cells were clearly visible under high power light microscopy. The relative positions of these two populations were determined by electron microscopy. Type I lymphocytes were attached to the surface of HEC and type II lymphocytes were flattened underneath. The transition from type I to type II was accompanied by a loss of surface microvilli and a redistribution of intracellular organelles. This suggested that lymphcoytes actively migrated across the HEC layer in this assay. The relationship between migrated lymphocytes and total adherent cells was determined by analysis of surface phenotype. Lymphocytes did not adhere randomly from the cell population plated. After 60 min there was an enrichment for B over T lymphocytes and the adherent T cell population was itself enriched for CD8+ cells over CD4+ cells. Migrated cells were, however, a random subpopulation of lymphocytes which adhered to HEC. This is clear evidence that migration was preceded by specific binding to HEC. Lymphocyte adhesion was independent of viable HEC showing that it was a passive event on the part of the endothelium. Lymphocyte migration was, however, completely dependent on viable high endothelium. We conclude that cultured HEC provide a biologically relevant, 3-dimensional matrix which supports the specific adhesion of lymphocytes and actively promotes lymphocyte migration. These observations suggest to us that cultured high endothelium provides a novel in vitro model for the study of lymphocyte migration into lymph nodes from the blood.