Autographa californica multiple nucleopolyhedrovirus core gene ac92 (p33) is required for efficient budded virus production
Autographa californica multiple nucleopolyhedrovirus core gene ac92 (p33) is required for efficient budded virus production
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DOI：
10.1016/j.virol.2010.09.023
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发表时间：
2011-01-05
期刊：
影响因子：
3.7
通讯作者：
Theilmann, David A.
中科院分区：
文献类型：
--
作者：
Nie, Yingchao;Fang, Minggang;Theilmann, David A.
Autographa call：图尼卡多核多角体病毒（AcMNPV）ac92是编码与闭塞衍生病毒（ODV）相关的蛋白质的核心基因，结合人P53，并且还具有黄素腺嘌呤二核苷酸连接的巯基氧化酶活性，但其在病毒生命周期中的作用尚不清楚。为了确定ac92功能，产生缺失病毒（vAc（92KO）），并且转染的Sf9细胞显示vAc（92KO）感染主要限于单细胞，并且出芽病毒（BV）滴度降低超过99.99%。然而，病毒DNA复制不受影响，并且在vAc（92KO）转染的细胞中的闭塞体的发展证明进展到病毒感染的非常晚期。AC92定位于细胞质和细胞核，并与BV以及ODV相关。在BV中，在BV包膜和核衣壳组分中检测到AC 92。最后，表明来自组II型杆状病毒Mamestra configurata NPV maco96的ac92同源物只能部分拯救vAc（92KO）。皇冠版权所有（C）2010由爱思唯尔公司出版。All rights reserved.
Autographa call:Tunica multiple nucleopolyhedrovirus (AcMNPV) ac92 is a core gene encoding a protein associated with occlusion derived virus (ODV), binds human P53 and also has Flavin adenine dinucleotide linked sulfhydryl oxidase activity but its role in the virus life cycle is not known. To determine ac92 function a deletion virus (vAc(92KO)) was generated and transfected Sf9 cells revealed that vAc(92KO) infection was restricted primarily to single cells and budded virus (BV) titer was reduced over 99.99%. However, viral DNA replication was unaffected and development of occlusion bodies in vAc(92KO)-transfected cells evidenced progression to very late phases of viral infection. AC92 localized to both the cytoplasm and nucleus, and was also associated with BV as well as ODV. In BV AC92 was detected in BV envelope and nucleocapsid fractions. Finally it was shown that the ac92 homologue from the Group II alphabaculovirus Mamestra configurata NPV maco96 could only partially rescue vAc(92KO). Crown Copyright (C) 2010 Published by Elsevier Inc. All rights reserved.