Magnetic self-assembly of toroidal hepatic microstructures for micro-tissue fabrication
Magnetic self-assembly of toroidal hepatic microstructures for micro-tissue fabrication
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DOI：
10.1088/1748-605x/ab8487
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发表时间：
2020-03
影响因子：
4
通讯作者：
Masaru Takeuchi;Masaki Iriguchi;M. Hattori;Eunhye Kim;A. Ichikawa;Y. Hasegawa;Qiang Huang;T. Fukuda
中科院分区：
文献类型：
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作者：
Masaru Takeuchi;Masaki Iriguchi;M. Hattori;Eunhye Kim;A. Ichikawa;Y. Hasegawa;Qiang Huang;T. Fukuda
在这项研究中，我们开发了一种利用磁性自组装将肝脏微结构组装成管状结构的方法，用于体外三维微组织制造。为此，利用微图案化电沉积方法制备了具有环形形状的生物相容水凝胶。用铁氧体颗粒包覆了制备的环形水凝胶微胶囊，并用聚L赖氨酸膜包裹了微胶囊。然后用3T的磁场对微胶囊进行磁化，并使用磁性自组装过程进行组装。在电沉积过程中，肝细胞被困在微囊内，并被培养以构建组织样结构。然后，磁化的环形微结构自动组装成管状结构。采用振动强化装配工艺，并对振动速度进行了实验优化，实现了较长筒体结构的高速装配。用粒子图像测速仪测量了摇动过程中碟子内部的流动速度。评估肝功能以检查磁化铁氧体颗粒对微观结构的副作用。综上所述，我们的研究结果表明，所开发的方法可以实现大量微结构的高速组装，形成组织状的肝脏结构。
In this study, we developed a procedure for assembling hepatic microstructures into tube shapes using magnetic self-assembly for in vitro 3D micro-tissue fabrication. To this end, biocompatible hydrogels, which have a toroidal shape, were made using the micro-patterned electrodeposition method. Ferrite particles were used to coat the fabricated toroidal hydrogel microcapsules using a poly-L-lysine membrane. The microcapsules were then magnetized with a 3 T magnetic field, and assembled using a magnetic self-assembly process. During electrodeposition, hepatic cells were trapped inside the microcapsules, and they were cultured to construct tissue-like structures. The magnetized toroidal microstructures then automatically assembled to form tube structures. Shaking was used to enhance the assembly process, and the shaking speed was experimentally optimized to achieve the high-speed assembly of longer tube structures. The flow velocity inside the dish during shaking was measured by particle image velocimetry. Hepatic functions were evaluated to check for side-effects of the magnetized ferrite particles on the microstructures. Collectively, our findings indicated that the developed method can achieve the high-speed assembly of a large number of microstructures to form tissue-like hepatic structures.