Distinct Root Microbial Communities in Nature Farming Rice Harbor Bacterial Strains With Plant Growth-Promoting Traits
Distinct Root Microbial Communities in Nature Farming Rice Harbor Bacterial Strains With Plant Growth-Promoting Traits
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DOI：
10.3389/fsufs.2020.629942
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发表时间：
2021-01
期刊：
影响因子：
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通讯作者：
Grace Flavyeliz Sinong;M. Yasuda;Y. Nara;Chol Gyu Lee;K. Dastogeer;H. Tabuchi;H. Nakai;Salem Djed
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文献类型：
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作者：
Grace Flavyeliz Sinong;M. Yasuda;Y. Nara;Chol Gyu Lee;K. Dastogeer;H. Tabuchi;H. Nakai;Salem Djed
自然农业系统是指不使用化肥和杀虫剂种植作物的生态农业实践。为了解与自然耕作系统相关的根系微生物群落的多样性和功能，我们比较了自然耕作条件下与常规耕作条件下水稻根系微生物群落。高通量扩增子分析表明，在未施肥的自然耕作条件下，根系微生物组的丰度和多样性高于常规耕作条件。化肥的施用降低了一些对植物生长和健康至关重要的有益类群的微生物多样性和丰度。随后，我们从自然耕作条件下生长的水稻根系中分离并鉴定了46种内生和附生细菌，并检测了它们的植物生长促进活性。选择6个潜在分离株在不溶性含磷和含钾培养基上进行植物生长评价。大部分分离株均能促进水稻生长，其中韩国假单胞菌AEPR1在不溶性含磷和含钾培养基中均能显著促进水稻生长。我们的数据表明，自然耕作系统创造了一个独特的根系微生物群，相对而言更多样化，并支持植物在低投入耕作方式下的生长。这些潜在的分离物在可持续农业中具有潜在的应用价值。
A nature farming system is an ecological farming practice that entails cultivating crops without using chemical fertilizers and pesticides. To understand the diversity and functions of root microbiomes associated with nature farming systems, we compared the root microbial community of rice under nature farming conditions with those under conventional farming conditions. High-throughput amplicon analysis demonstrated a higher abundance and greater diversity of the root microbiome under unfertilized nature farming conditions than under conventional conditions. The application of chemical fertilizers reduced the microbial diversity and abundance of some beneficial taxa important for plant growth and health. Subsequently, we isolated and identified 46 endo- and epiphytic bacteria from rice roots grown under nature farming conditions and examined their plant growth-promoting activity. Six potential isolates were selected for plant growth assessment in insoluble P- and K-containing media. Most of the isolates promoted rice growth, and Pseudomonas koreensis AEPR1 was able to enhance rice growth significantly in both insoluble P- and K-containing media. Our data indicated that nature farming systems create a distinct root microbiome that is comparatively more diverse and supports plant growth under low-input cultivation practices than under conventional practices. The potential isolates could be exploited as sources with potential applications in sustainable agriculture.