Time course of protein synthesis-dependent phase of olfactory memory in the cricket Gryllus bimaculatus
Time course of protein synthesis-dependent phase of olfactory memory in the cricket Gryllus bimaculatus
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DOI：
10.2108/zsj.20.409
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发表时间：
2003-04-01
影响因子：
0.9
通讯作者：
Mizunami, M
中科院分区：
文献类型：
--
作者：
Matsumoto, Y;Noji, S;Mizunami, M
蟋蟀Gryllus bimaculatus形成稳定的嗅觉记忆，几乎持续一生。作为阐明蟋蟀嗅觉学习和记忆保持的细胞机制的第一步，我们通过将蛋白质合成抑制剂放线菌酮（CHX）注射到头囊中来研究记忆保持对从头脑蛋白质合成的依赖性。注射CHX抑制H-3-亮氨酸掺入脑蛋白质的> 90%，持续3 hr. Crickets被训练将薄荷气味与水（奖励）和香草气味与盐水溶液（非奖励）联系起来，并在训练前或训练后的不同时间注射CHX。在训练后2小时、1天和4天测试他们的气味偏好。训练后2小时的记忆保持不受CHX注射的影响。然而，当在训练前1小时或训练后1小时或6小时注射CHX时，训练后1天和4天的保持水平降低。为了研究CHX敏感记忆阶段发展的时间过程，在训练后1小时注射CHX的蟋蟀在训练后2至12小时的不同时间进行测试。保持水平不受影响的训练后4小时，但显着降低5小时后，训练，CHX敏感的记忆阶段逐渐发展在接下来的几个小时。CHX将蟋蟀的嗅觉记忆分为两个阶段：早期蛋白质合成非依赖性阶段（< 4小时）和晚期蛋白质合成依赖性阶段（> 5小时）。
The cricket Gryllus bimaculatus forms a stable olfactory memory that lasts for practically a lifetime. As a first step to elucidate the cellular mechanisms of olfactory learning and memory retention in crickets, we studied the dependency of memory retention on the de novo brain protein synthesis by injecting the protein synthesis inhibitor cycloheximide (CHX) into the head capsule. Injection of CHX inhibited H-3-leucine incorporation into brain proteins by > 90% for 3 hr. Crickets were trained to associate peppermint odor with water (reward) and vanilla odor with saline solution (non-reward) and were injected with CHX before or at different times after training. Their odor preferences were tested at 2 hr, 1 day and 4 days after training. Memory retention at 2 hr after training was unaffected by CHX injection. However, the level of retention at 1 day and 4 days after training was lowered when CHX was injected 1 hour before training or at 1 hr or 6 hr after training. To study the time course of the development of CHX-sensitive memory phase, crickets that had been injected with CHX at 1 hr after training were tested at different times from 2 to 12 hr after training. The level of retention was unaffected up to 4 hr after training but significantly lowered at 5 hr after training, and the CHX-sensitive memory phase developed gradually during the next several hours. CHX dissociates two phases of olfactory memory in crickets: earlier protein synthesis-independent phase (< 4 hr) and later (> 5 hr) protein synthesis-dependent phase.