Isoflurane strongly affects the diffusion of intracellular metabolites, as shown by 1H nuclear magnetic resonance spectroscopy of the monkey brain
Isoflurane strongly affects the diffusion of intracellular metabolites, as shown by 1H nuclear magnetic resonance spectroscopy of the monkey brain
复制标题
DOI：
10.1038/sj.jcbfm.9600353
复制
发表时间：
2007-03-01
影响因子：
6.3
通讯作者：
Lebon, Vincent
中科院分区：
文献类型：
--
作者：
Valette, Julien;Guillermier, Martine;Lebon, Vincent
异氟烷是一种常用于动物研究的挥发性麻醉剂。特别是，扩散核磁共振（NMR）光谱经常在异氟烷麻醉下进行。然而，已知异氟烷会影响脂质双层的相变，可能导致代谢物渗透性增加。由此导致的限制减少可能会影响代谢物表观扩散系数（ADC）。在目前的工作中，异氟烷剂量对代谢物ADC的影响进行了评估，使用扩散张量光谱在猴脑。对于检测到的五种细胞内代谢物，当异氟烷剂量从1%变化到2%时，ADC显示出显著增加：肌肌醇为13% +/- 8%，总N-乙酰基-天冬氨酸为14% +/- 13%，谷氨酸为20% +/- 18%，总肌酸为27% +/- 7%，总胆碱为53% +/- 17%。对5种不同代谢物经历的ADC变化的详细分析支持在高异氟烷剂量下促进亚细胞结构之间的代谢物交换。这项工作强烈支持了体内代谢物扩散在长扩散时间内显着限制在亚细胞结构中的想法，并为解释扩散核磁共振光谱测量的ADC值提供了新的见解。
Isoflurane is a volatile anesthetic commonly used for animal studies. In particular, diffusion nuclear magnetic resonance ( NMR) spectroscopy is frequently performed under isoflurane anesthesia. However, isoflurane is known to affect the phase transition of lipid bilayer, possibly resulting in increased permeability to metabolites. Resulting decreased restriction may affect metabolite apparent diffusion coefficient ( ADC). In the present work, the effect of isoflurane dose on metabolite ADC is evaluated using diffusion tensor spectroscopy in the monkey brain. For the five detected intracellular metabolites, the ADC exhibits a significant increase when isoflurane dose varies from 1% to 2%: 13% +/- 8% for myo- inositol, 14% +/- 13% for total N- acetyl- aspartate, 20% +/- 18% for glutamate, 27% +/- 7% for total creatine and 53% +/- 17% for total choline. Detailed analysis of ADC changes experienced by the five different metabolites argues in favor of facilitated metabolite exchange between subcellular structures at high isoflurane dose. This work strongly supports the idea of metabolite diffusion in vivo being significantly restricted in subcellular structures at long diffusion time, and provides new insights for interpreting ADC values as measured by diffusion NMR spectroscopy.