DNA barcoding analysis of Coleoidea (Mollusca: Cephalopoda) from Chinese waters
DNA barcoding analysis of Coleoidea (Mollusca: Cephalopoda) from Chinese waters
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DOI：
10.1111/j.1755-0998.2012.03118.x
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发表时间：
2012-05-01
影响因子：
7.7
通讯作者：
Li, Qi
中科院分区：
文献类型：
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作者：
Dai, Lina;Zheng, Xiaodong;Li, Qi
鞘形动物属于头足类动物，在大多数海洋中无论是在生态层面还是在商业层面都占据着重要地位。然而，当标本在采集过程中受到严重破坏或存在密切相关的类群时，一些鞘科物种很难用传统的形态识别方法进行区分。作为快速物种分配的有用工具，DNA 条形码可能为胶状体鉴定提供巨大潜力。在这里，我们使用两个线粒体片段，细胞色素c氧化酶I和大核糖体亚基（16S rRNA），来评估是否可以通过DNA条形码技术来鉴定占中国鞘类动物群约三分之一的34种鞘类动物。评估了成对的种内和种间距离，并通过新泽西州和贝叶斯分析估计了物种之间的关系。 Loliolus beka 内的高水平遗传分化导致种内和种间距离之间的重叠。在新泽西树和贝叶斯树中形成分化良好的分支的所有剩余物种对于两个片段来说是相同的。 Loliolus beka 拥有两个线粒体谱系，种内距离较高，表明存在隐秘物种。这项研究证实了 DNA 条形码在识别物种以及发现中国蝾螈的神秘多样性方面的功效。这也为鞘翅目其他生态和生物学研究奠定了基础。
Coleoids are part of the Cephalopoda class, which occupy an important position in most oceans both at an ecological level and at a commercial level. Nevertheless, some coleoid species are difficult to distinguish with traditional morphological identification in cases when specimens are heavily damaged during collection or when closely related taxa are existent. As a useful tool for rapid species assignment, DNA barcoding may offer significant potential for coleoid identification. Here, we used two mitochondrial fragments, cytochrome c oxidase I and the large ribosomal subunit (16S rRNA), to assess whether 34 coleoids accounting for about one-third of the Chinese coleoid fauna could be identified by DNA barcoding technique. The pairwise intra- and interspecific distances were assessed, and relationships among species were estimated by NJ and Bayesian analyses. High levels of genetic differentiation within Loliolus beka led to an overlap between intra- and interspecific distances. All remaining species forming well-differentiated clades in the NJ and Bayesian trees were identical for both fragments. Loliolus beka possessed two mitochondrial lineages with high levels of intraspecific distances, suggesting the occurrence of cryptic species. This study confirms the efficacy of DNA barcoding for identifying species as well as discovering cryptic diversity of Chinese coleoids. It also lays a foundation for other ecological and biological studies of Coleoidea.