TRANSCRIPTION FACTOR NUCLEAR FACTOR-I PROTEINS FORM STABLE HOMODIMERS AND HETERODIMERS
TRANSCRIPTION FACTOR NUCLEAR FACTOR-I PROTEINS FORM STABLE HOMODIMERS AND HETERODIMERS
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DOI：
10.1016/0014-5793(94)00585-0
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发表时间：
1994-07-04
期刊：
影响因子：
3.5
通讯作者：
SIPPEL, AE
中科院分区：
文献类型：
--
作者：
KRUSE, U;SIPPEL, AE
核因子I （NFI）蛋白是真核生物DNA结合蛋白的一个大家族。它们参与病毒和细胞方面的转录调控，它们能够刺激腺病毒开始复制。使用由四种不同的鸡NFI基因编码的体外翻译的NFI蛋白，我们已经检测到所有测试组合的同二聚体和异二聚体。异源二聚体的形成严重依赖于共翻译，这表明在没有DNA的情况下二聚体的形成是稳定的。NFI蛋白的不受限制的异源二聚化，除了基因多样性和选择性剪接之外，增加了该蛋白家族的另一层次的多样性。
Nuclear factor I (NFI) proteins constitute a large family of eukaryotic DNA binding proteins. They are involved in viral and cellular aspects of transcriptional regulation and they are capable of stimulating adenovirus initiation of replication. Using in vitro translated NFI proteins encoded by four different chicken NFI genes, we have detected homodimers as well as heterodimers for all combinations tested. The formation of heterodimers was critically dependent on cotranslation, indicating stable dimer formation in the absence of DNA. The unrestricted heterodimerization of NFI proteins adds, beside gene diversity and alternative splicing, another level of diversity to this protein family.