Radiosensitization of two murine rumours with mild temperature hyperthermia and carbogen breathing
Radiosensitization of two murine rumours with mild temperature hyperthermia and carbogen breathing
复制标题
DOI：
10.1080/095530099139467
复制
发表时间：
1999-10-01
影响因子：
2.6
通讯作者：
Song, CW
中科院分区：
文献类型：
--
作者：
Griffin, RJ;Okajima, K;Song, CW
目的：为探讨局部41.5 ℃ 60 min低温热疗（MTH）和呼吸Carbogen对肿瘤放射敏感性的影响，材料和方法：采用C3 H小鼠FSall纤维肉瘤和A/J小鼠SCK乳腺癌模型。使用体内/体外肿瘤切除试验确定各种治疗对肿瘤细胞存活的影响，并计算放射生物学缺氧分数。肿瘤的辐射敏感性进行了测试与肿瘤生长延迟assay.Results：放射性缺氧细胞分数（HF）在控制FSall和SCK肿瘤分别为0.45和0.78，这些值下降到0.12 FSall肿瘤和0.22 SCK肿瘤时，立即确定后，肿瘤用MTH治疗。应用Carbogen呼吸后，FSall肿瘤的KF为0.32，SCK肿瘤的KF为0.33。当肿瘤用MTH治疗，动物呼吸carbogen时，HF在FSall和SCK谣言中都降低到0.03-0.04。单独使用MTH治疗对肿瘤生长只有很小的影响，但在照射谣言之前使用MTH治疗显着增加了辐射引起的肿瘤生长延迟。Carbogen呼吸温和地改善了辐射诱导的肿瘤生长延迟，而MTH治疗和Carbogen呼吸的组合造成了最大的增加，辐射诱导的肿瘤生长delay.Conclusions：MTH治疗单独和结合Carbogen呼吸大幅增加肿瘤的辐射反应可能通过增加肿瘤的氧合状态。
Purpose: To test the individual and combined effects of local mild temperature hyperthermia (MTH) at 41.5 degrees C for 60 min and carbogen breathing on tumour radiosensitivity.Materials and methods: The FSall fibrosarcoma of C3H mice and the SCK mammary carcinoma of A/J mice were used. The effect of various treatments on tumour cell survival was determined using the in vivo/in vitro tumour excision assay, and the radiobiological hypoxic fraction was calculated. The tumour radiation sensitivity was tested with the tumour growth delay assay.Results: The radiobiological hypoxic cell fraction (HF) in control FSall and SCK tumours was 0.45 and 0.78, respectively, and these values decreased to 0.12 in FSall tumours and 0.22 in SCK tumours when determined immediately after the tumours were treated with MTH. The KF was 0.32 in FSall tumours and 0.33 in SCK tumours after carbogen breathing was applied. When tumours were treated with MTH and the animals breathed carbogen the HF decreased to 0.03-0.04 in both FSall and SCK rumours. MTH treatment alone had only a small effect on tumour growth, but MTH treatment applied before irradiating the rumours significantly increased the radiation-induced tumour growth delay. Carbogen breathing modestly improved the radiation-induced tumour growth delay while the combination of MTH treatment and carbogen breathing caused the largest increase in radiation-induced tumour growth delay.Conclusions: MTH treatment alone and combined with carbogen breathing substantially increased the tumour radiation response probably through an increase in the tumour oxygenation status.