Metabolism and effects on platelet function of the purified eicosapentaenoic and docosahexaenoic acids in humans.
Metabolism and effects on platelet function of the purified eicosapentaenoic and docosahexaenoic acids in humans.
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纯化的二十碳五烯酸和二十二碳六烯酸在人体内的代谢及其对血小板功能的影响。
DOI：
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发表时间：
1985
影响因子：
15.9
通讯作者：
Peter C. Weber
中科院分区：
文献类型：
--
作者：
C. Schacky;Peter C. Weber
在一项随机交叉研究中，比较了志愿者服用6 g/d的二十碳五烯酸（EPA，C20：5 omega-3）或二十二碳六烯酸（DCHA，C22：6 omega-3）6 d后的代谢和对血小板功能的影响。掺入动力学显示，EPA出现在血浆游离脂肪酸和血浆磷脂4小时后，但没有纳入血小板磷脂酰胆碱和乙醇胺，直到第6天。这表明血小板脂肪酸组成不能立即反映周围血浆环境的组成，而是可以在巨核细胞成熟过程中确定。重要的是，EPA没有纳入血小板磷脂酰肌醇或丝氨酸在体内，从而反映了血小板磷脂的选择性生物合成。膳食EPA后，C22：5 omega-3增加血浆和血小板磷脂。相反，DCHA水平没有改变。摄入DCHA后，血浆磷脂中C20：5 omega-3浓度升高，这意味着发生了逆转。这些发现表明，膳食DCHA可以作为EPA的来源。在这项短期研究中，EPA和DCHA的摄入导致血小板对胶原蛋白的反应减少。ADP的反应显着降低，只有DCHA。在EPA或DCHA后，血栓烷形成在来自凝结全血的血清中不变，通过免疫反应性2，3-dinor血栓烷B2, 3的排泄测量的体内总合成也不变。我们的结论是，DCHA降低血小板反应性，有助于抗血栓形成的ω-3脂肪酸丰富的鱼油摄入，其中DCHA是一个主要组成部分。
Metabolism and effects on platelet function of 6 g/d for 6 d of either eicosapentaenoic acid (EPA, C20:5 omega-3) or docosahexaenoic acid (DCHA, C22:6 omega-3) in volunteers were compared in a randomized crossover study. Incorporation kinetics revealed that EPA appeared in plasma free fatty acids and plasma phospholipids after 4 h, but was not incorporated into platelet phosphatidylcholine and -ethanolamine until day 6. This indicates that platelet fatty acid composition does not immediately reflect that of the surrounding plasma milieu, but rather may be determined during megakaryocyte maturation. Importantly, EPA was not incorporated into platelet phosphatidylinositol or -serine in vivo, thus reflecting selective biosynthesis of platelet phospholipids. After dietary EPA, C22:5 omega-3 increased in plasma and platelet phospholipids. In contrast, DCHA-levels were unaltered. After DCHA-ingestion, C20:5 omega-3 concentrations rose in plasma phospholipids, implying that retroconversion took place. These findings indicate that dietary DCHA can serve as a source of EPA. During this short-term study, ingestion of both EPA and DCHA resulted in reduced platelet aggregation in response to collagen. The response to ADP was lowered significantly only by DCHA. After either EPA or DCHA, thromboxane formation was unchanged in serum derived from clotted whole blood as was total in vivo synthesis measured by excretion of immunoreactive 2,3-dinor thromboxane B2/3. We conclude that DCHA reduces platelet responsiveness, contributing to the antithrombotic effects of omega-3 fatty acid-rich fish oil ingestion, of which DCHA is a major component.
二十二碳六烯酸（DHA）对花生四烯酸代谢和血小板功能的影响。
DOI：
10.1016/0006-291x(83)91235-4
发表时间：
1983
影响因子：
3.1
作者：
Rao,GH;Radha,E;White,JG
通讯作者：
White,JG
DOI：
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发表时间：
1985
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Weiner,TW;Sprecher,H
通讯作者：
Sprecher,H