Double peroxidase and histone acetyltransferase AgTip60 maintain innate immune memory in primed mosquitoes
Double peroxidase and histone acetyltransferase AgTip60 maintain innate immune memory in primed mosquitoes
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DOI：
10.1073/pnas.2114242118
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发表时间：
2021-11-02
影响因子：
11.1
通讯作者：
Barillas-Mury, Carolina
中科院分区：
文献类型：
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作者：
Gomes, Fabio M.;Tyner, Miles D. W.;Barillas-Mury, Carolina
冈比亚按蚊的免疫启动是由血细胞分化因子（HDF）的全身释放介导的，HDF是脂氧素A4与脂质载体Evokin结合的复合物。HDF增加循环粒细胞的比例，增强蚊子的细胞免疫力。在这里，我们发现Evokin存在于血细胞和脂肪体细胞中，并且在免疫引发后信使RNA（mRNA）表达显著增加。双过氧化物酶（DBLOX）存在于昆虫中，但不存在于脊椎动物中，是HDF合成所必需的。DBLOX在脂肪体组织中的卵母细胞中高度表达，并且这些细胞在引发的蚊子中数量增加。我们提供的直接证据表明，组蛋白乙酰转移酶AgTip60（AGAP001539）也是必要的，持续增加的卵母细胞数量，HDF合成，免疫启动。我们建议，卵母细胞可能作为一个人口的细胞，重新编程，编排和维持一个广泛的，系统的，持久的状态，增强免疫监视在引发蚊子。
Immune priming in Anopheles gambiae is mediated by the systemic release of a hemocyte differentiation factor (HDF), a complex of lipoxin A4 bound to Evokin, a lipid carrier. HDF increases the proportion of circulating granulocytes and enhances mosquito cellular immunity. Here, we show that Evokin is present in hemocytes and fat-body cells, and messenger RNA (mRNA) expression increases significantly after immune priming. The double peroxidase (DBLOX) enzyme, present in insects but not in vertebrates, is essential for HDF synthesis. DBLOX is highly expressed in oenocytes in the fat-body tissue, and these cells increase in number in primed mosquitoes. We provide direct evidence that the histone acetyltransferase AgTip60 (AGAP001539) is also essential for a sustained increase in oenocyte numbers, HDF synthesis, and immune priming. We propose that oenocytes may function as a population of cells that are reprogrammed, and orchestrate and maintain a broad, systemic, and long-lasting state of enhanced immune surveillance in primed mosquitoes.