Disruption of ADAM3 Impairs the Migration of Sperm into Oviduct in Mouse
Disruption of ADAM3 Impairs the Migration of Sperm into Oviduct in Mouse
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DOI：
10.1095/biolreprod.108.074021
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发表时间：
2009-07-01
影响因子：
3.6
通讯作者：
Okabe, Masaru
中科院分区：
文献类型：
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作者：
Yamaguchi, Ryo;Muro, Yuko;Okabe, Masaru
已知来自四种不同基因破坏小鼠品系（calmegin [Clgn]、Adam 1a、Adam 2和Ace）的精子具有缺陷的透明带结合能力。此外，据报道，所有这些小鼠品系的精子表现出另一种常见的表型，即迁移到输卵管中受损，尽管在性交后子宫中发现了大量精子。另一方面，据报道，来自Adam 3-破坏的小鼠系的精子与精子的结合能力有缺陷，但能够进入输卵管。为了阐明这一差异，我们用顶体蛋白-绿色荧光蛋白作为标记物观察精子，精确地研究了ADAM 3缺失精子向输卵管的迁移。结果，与以前的观察相反，证明了Adam 3破坏的精子在性交后不能迁移到输卵管中。最终表明，在5个不同的基因破坏小鼠品系中，5个表现为精子与透明质酸结合受损的表型伴随着精子迁移到输卵管的能力丧失。这表明这两种现象之间的密切关系，也表明精子迁移到输卵管是受精的关键步骤。
Sperm from four different gene-disrupted mouse lines (calmegin [Clgn], Adam1a, Adam2, and Ace) are known to have defective zona-binding ability. Moreover, it is also reported that the sperm from all of these mouse lines exhibit another common phenotype of impaired migration into oviduct despite the large number of sperm found in uterus after coitus. On the other hand, the sperm from the Adam3-disrupted mouse line was reported to have defects in binding ability to zona, but were able to move into the oviduct. In order to clarify the difference, we investigated the migration of ADAM3-null sperm into oviduct precisely by visualizing the sperm by using acrosin-green fluorescent protein as a tag. As a result, in contrast to previous observations, it was demonstrated that the Adam3-disrupted sperm were unable to migrate into the oviduct after coitus. It was ultimately shown that, in five out of five different gene-disrupted mouse lines, the phenotype of impaired sperm binding to zona pellucida was accompanied by the loss of ability of sperm to migrate into the oviduct. This indicates a close relationship between the two phenomena, and also that sperm migration into the oviduct is a crucial step for fertilization.