Is biofilm accumulation on endoscope tubing a contributor to the failure of cleaning and decontamination?
Is biofilm accumulation on endoscope tubing a contributor to the failure of cleaning and decontamination?
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DOI：
10.1016/j.jhin.2004.06.023
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发表时间：
2004-11-01
影响因子：
6.9
通讯作者：
Cossart, Y
中科院分区：
文献类型：
--
作者：
Pajkos, A;Vickery, K;Cossart, Y
我们预测，与液体接触的内窥镜管表面会形成生物膜，可能很难通过目前的清洗程序去除。它的存在可能会保护微生物不受消毒作用的影响，并导致在重复使用之前无法进行净化。从送往内窥镜服务中心的13个内窥镜取出的管子样本通过扫描电子显微镜检查了生物膜和细菌的存在。所有被测试的样本上都有生物沉积物。13个器械中有5个在抽吸/活组织检查通道上存在生物膜(细菌+胞外多糖基质)，其中一个器械上的生物膜非常广泛。细菌和微菌落经常但不一定与油管表面缺陷有关。所有被检查的12个空气/水通道都显示出生物膜，这在9个样本上都很广泛。常规清洁程序不能可靠地从内窥镜通道中去除生物膜，这可能解释了尽管很好地遵守了感染控制指南，但在实践中遇到的去污染意外失败。(C)2004年医院感染学会。爱思唯尔有限公司出版。保留所有权利。
We predicted that biofilm would form on surfaces of endoscope tubing in contact with fluids, and may be difficult to remove by current washing procedures. Its presence may protect micro-organisms from disinfectant action and contribute to failure of decontamination prior to reuse. Tubing samples removed from 13 endoscopes that had been sent to an endoscope-servicing centre were examined for the presence of biofilm and bacteria by scanning electron microscopy. Biological deposits were present on all samples tested. Biofilm (bacteria plus exopolysaccharides matrix) was present on the suction/biopsy channels of five of 13 instruments, and was very extensive on one of these. Bacteria and microcolonies were often but not necessarily associated with surface defects on the tubing. All 12 air/water channels examined showed biofilm, and this was extensive on nine samples. Routine cleaning procedures do not remove biofilm reliably from endoscope channels, and this may explain the unexpected failure of decontamination encountered in practice despite good adherence to infection control guidelines. (C) 2004 The Hospital Infection Society. Published by Elsevier Ltd. All rights reserved.