Genome-wide analysis and identification of TIR-NBS-LRR genes in Chinese cabbage (Brassica rapa ssp pekinensis) reveal expression patterns to TuMV infection

Genome-wide analysis and identification of TIR-NBS-LRR genes in Chinese cabbage (Brassica rapa ssp pekinensis) reveal expression patterns to TuMV infection
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DOI:
10.1016/j.pmpp.2015.04.001
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发表时间:
2015-04-01
影响因子:
2.7
通讯作者:
Hou, Xilin
Hou, Xilin
中科院分区:
农林科学3区
文献类型:
--
作者:
Lv, Shanwu;Changwei, Z.;Hou, Xilin

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TIR-NBS-LRR (TNL) 基因极大地影响植物的生长发育。在大白菜(Brassica rapa ssp. pekinensis)中鉴定并表征了 90 个 TNL 型基因。组织表达谱揭示了不同组织中不同的表达水平。 qRT-PCR分析揭示了芜菁花叶病毒(TuMV)攻击后69个基因的表达模式:42个基因上调,11个基因下调;基因根据其不同的表达模式进行分组。 16 个候选基因被鉴定为对 TuMV 感染有反应。这项研究提供了有关大白菜对TuMV反应的抗性基因的信息,并进一步加深了对白菜作物抗性机制的理解。 (C) 2015 Elsevier Ltd. 保留所有权利。
TIR-NBS-LRR (TNL) genes greatly affect plant growth and development. Ninety TNL-type genes were identified and characterized in Chinese cabbage (Brassica rapa ssp. pekinensis). Tissue-expression profiling revealed different expression levels in different tissues. qRT-PCR analysis revealed the expression patterns of 69 genes challenged by Turnip mosaic virus (TuMV): 42 genes were up-regulated, and 11 genes down-regulated; genes were grouped according to their different expression patterns. Sixteen candidate genes were identified as responding to TuMV infection. This study supplies information on resistance genes involved in Chinese cabbage's response against TuMV, and furthers the understanding of resistance mechanisms in B. rapa crops. (C) 2015 Elsevier Ltd. All rights reserved.