Architecture and distribution of human corneal nerves
Architecture and distribution of human corneal nerves
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DOI：
10.1136/bjo.2009.173799
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发表时间：
2010-06-01
影响因子：
4.1
通讯作者：
Dua, Harminder S.
中科院分区：
文献类型：
--
作者：
Al-Aqaba, Mouhamed A.;Fares, Usama;Dua, Harminder S.
方法对21例正常人角膜、2例眼库角膜-巩膜扣、2例眼库角膜-巩膜缘和3例圆锥角膜术后标本进行解剖学研究。用Karnovsky & Roots直接染色乙酰胆碱酯酶（AchE. Results）硫代胆碱修饰法对人角膜全标本进行胆碱酯酶染色。结果发现约44条粗神经束以相对均匀的分布方式进入人角膜利姆布斯周围，并随机向角膜中央移动。在中周边区，前基质神经表现出特征性的出芽和分支模式。在穿过Bowman区后，注意到它们终止于球状增厚，从该球状增厚中产生多个基底下神经。穿孔部位主要位于中周边角膜。基底下神经的方向主要是垂直于穿孔部位的起源。来自各个方向的神经汇聚到下中央角膜，形成一个特征性的顺时针螺纹pattern.Conclusions这项研究提供了一个全面的帐户的架构和分布的神经在人类角膜。它调和了一些现有的信息，从其他方式的调查，并确定了一些新的功能，提供了一个更完整的图片角膜神经支配。
Aims To comprehensively study the gross anatomy of human corneal innervation.Methods Twenty-one specimens, including 12 normal human corneas from seven deceased patients, two eye-bank corneo-scleral buttons, two eye-bank corneo-scleral rims and five post-surgical specimens from three patients with keratoconus were studied. Corneal whole mounts were stained for cholinesterase enzyme using the Karnovsky & Roots direct colouring thiocholine modification of acetylcholinesterase (AchE) technique.Results Approximately 44 thick nerve bundles were found to enter the human cornea in a relatively equal distribution round the limbus and move randomly towards the central cornea. At the mid-peripheral zone, anterior stromal nerves showed a characteristic budding and branching pattern. After passing through Bowman's zone they were noted to terminate into bulb-like thickenings from which multiple sub-basal nerves arose. The perforation sites were predominantly located in the mid-peripheral cornea. The orientation of sub-basal nerves was mainly vertical at their origin from the perforation sites. Nerves from all directions converged towards the infero-central cornea to form a characteristic clockwise whorl pattern.Conclusions This study provides a comprehensive account of the architecture and distribution of nerves in the human cornea. It reconciles some of the existing information obtained from other modalities of investigation and identifies some novel features that provide a more complete picture of corneal innervation.