Influence of acinar tissue contamination on encapsulated pancreatic islets - Morphological and functional studies
Influence of acinar tissue contamination on encapsulated pancreatic islets - Morphological and functional studies
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DOI：
10.1097/00007890-199705270-00032
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发表时间：
1997-05-27
期刊：
影响因子：
6.2
通讯作者：
Pinget, M
中科院分区：
文献类型：
--
作者：
Kessler, L;Jesser, C;Pinget, M
背景本研究旨在探讨腺泡污染对人工膜（AN 69）包裹胰岛的影响。通过添加腺泡组织（比例，1：1）污染纯的、手工挑选的胰岛。在大鼠腹腔内培养或植入10 d后，对纯胰岛（n=12）和污染胰岛（n=12）的形态学和胰岛素释放进行了评价。植入后，被包裹的胰岛，无论其纯度如何，都被完全改变，而培养的胰岛的形态学方面仅在没有腺泡组织污染的情况下保持完整。这种污染引起胰岛素释放的刺激指数显著降低。新鲜胰岛的刺激指数下降了42%，培养和植入胰岛的刺激指数分别下降了52%和34%，而胰岛素的基础释放没有改变。结论。腺泡组织被证明是有害的封装植入和培养的胰岛。
Background. The present study concerns the influence of acinar contamination on pancreatic islets encapsulated in an artificial membrane (AN 69).Methods. Pure, handpicked pancreatic islets were contaminated by the addition of acinar tissue (ratio, 1:1). The morphological aspect and insulin release of both pure (n=12) and contaminated (n=12) encapsulated islets were assessed after 10 days of culture or implantation in the peritoneal cavity of rats.Results. After implantation, the encapsulated islets, irrespective of their purity, were totally altered, whereas the morphological aspect of the cultivated isIets remained intact only in the absence of acinar tissue contamination. This contamination induced a significant decrease in the stimulation index of insulin release. The stimulation index decreased by 42% for fresh islets and by 52% and 34% for cultivated and implanted islets, respectively, without modification in their basal release of insulin.Conclusions. The acinar tissue proved detrimental to the encapsulated implanted and cultivated islets.