The fractalkine receptor but not CCR2 is present on microglia from embryonic development throughout adulthood.
The fractalkine receptor but not CCR2 is present on microglia from embryonic development throughout adulthood.
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DOI：
10.4049/jimmunol.1100421
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发表时间：
2012-01-01
期刊：
影响因子：
--
通讯作者：
Cardona AE
中科院分区：
文献类型：
--
作者：
Mizutani M;Pino PA;Saederup N;Charo IF;Ransohoff RM;Cardona AE
由于缺乏针对髓系亚群的特异性试剂，小胶质细胞在发育过程中难以追踪。为了进一步了解髓系谱系在发育过程中如何分化产生小胶质细胞，我们研究了在Cx3cr1 + / GFP - CCR2 + / RFP敲入荧光蛋白报告小鼠中CX3CR1和CCR2转录单元的激活情况。主要发现包括：1）CX3CR1 +细胞定位于主动脉 - 性腺 - 中肾（AGM）区域，并在胚胎第9.0天（E9.0）在卵黄囊和神经外胚层中可见；2）在胚胎第10.5天（E10.5），可观察到CX3CR1单阳性小胶质细胞穿透神经上皮；3）通过组织切片和流式细胞术分析，CX3CR1和CCR2可区分浸润的巨噬细胞与组织内驻留的监视性或活化的小胶质细胞。我们的结果支持卵黄囊作为小胶质前体来源的观点。我们提供了一种新的模型，用于监测小胶质细胞和髓系细胞在发育早期（E8.0 - E10.5）以及炎症状态下趋化因子受体表达的变化，这在哺乳动物组织中一直难以观察到。
Microglial cells are difficult to track during development due to the lack of specific reagents for myeloid sub-populations. To further understand how myeloid lineages differentiate during development to give rise to microglial cells, we investigated CX3CR1 and CCR2 transcription unit activation in Cx3cr1+/GFPCCR2+/RFP knock-in fluorescent protein reporter mice. The principal findings include: 1) CX3CR1+ cells localized to the AGM region, and visualized at E9.0 in the yolk sac and neuroectoderm, 2) At E10.5 CX3CR1 single positive microglial cells were visualized penetrating the neuroepithelium, 3) CX3CR1 and CCR2 distinguished infiltrating macrophages from resident surveillant or activated microglia within tissue sections and by flow cytometric analyses. Our results support the contribution of the yolk sac as source of microglial precursors. We provide a novel model to monitor chemokine receptor expression changes in microglia and myeloid cells early (E8.0-E10.5) in development and during inflammatory conditions, which have been challenging to visualize in mammalian tissues.
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影响因子：
20.3
作者：
King, Irah L.;Dickendesher, Travis L.;Segal, Benjamin M.
通讯作者：
Segal, Benjamin M.
影响因子：
5.3
作者：
Jung, S;Aliberti, J;Littman, DR
通讯作者：
Littman, DR
DOI：
10.1073/pnas.95.18.10896
发表时间：
1998-09-01
影响因子：
11.1
作者：
Harrison, JK;Jiang, Y;Feng, LL
通讯作者：
Feng, LL
影响因子：
9.3
作者：
Eltayeb S;Berg AL;Lassmann H;Wallström E;Nilsson M;Olsson T;Ericsson-Dahlstrand A;Sunnemark D
通讯作者：
Sunnemark D
影响因子：
25
作者：
Fuhrmann, Martin;Bittner, Tobias;Jung, Christian K. E.;Burgold, Steffen;Page, Richard M.;Mitteregger, Gerda;Haass, Christian;LaFerla, Frank M.;Kretzschmar, Hans;Herms, Jochen
通讯作者：
Herms, Jochen