High fat diet modulates inflammatory parameters in the heart and liver during acute Trypanosoma cruzi infection
High fat diet modulates inflammatory parameters in the heart and liver during acute Trypanosoma cruzi infection
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DOI：
10.1016/j.intimp.2018.08.036
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发表时间：
2018-11-01
影响因子：
5.6
通讯作者：
Talvani, Andre
中科院分区：
文献类型：
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作者：
Figueiredo, Vivian Paulino;Lopes Junior, Evandro Saraiva;Talvani, Andre
高脂饮食（HFD）可引发代谢和心血管疾病。克氏锥虫感染诱导能够影响重要器官如心脏和肝脏的结构和功能的进行性炎症表现。本研究旨在探讨HFD对T.克氏病毒VL-10。VL-10菌株具有向心性，并在小鼠中引起心肌炎。雄性C57 BL/6小鼠用（i）常规饮食（Reg）或（ii）HFD处理8周，之后每组中的小鼠用T.克鲁兹在感染后第30天对小鼠实施安乐死，并对肝脏和心脏进行组织学和酶谱分析以确定MMP-2活性和IL-10、TNF、CCL 2和CCL 5的血浆水平。t.克氏病毒感染的HFD动物具有较高的寄生虫血症、LDL和总胆固醇水平。无论饮食，血浆水平的所有炎症介质和心脏MMP-2活性升高感染的小鼠与低血浆水平的瘦素。HFD动物表现为微小泡和大泡性肝脂肪变性，而心脏白细胞浸润主要见于T。克鲁兹感染的小鼠。我们的研究结果表明，HFD促进更高的循环T。cruzi负荷和心脏和肝脏免疫发病机制的实验模型中使用T.克鲁兹
The high fat diet (HFD) can trigger metabolic and cardiovascular diseases. Trypanosome cruzi infection induces progressive inflammatory manifestations capable to affect the structure and the function of important organs such as the heart and liver. Here we aimed to investigate the effects of a HFD on the immune response and matrix metalloproteinase (MMP) activities during acute infection with the T. cruzi strain VL-10. The VL-10 strain has cardiac tropism and causes myocarditis in mice. Male C57BL/6 mice were treated with either: (i) regular diet (Reg) or (ii) HFD for 8 weeks, after which mice in each group were infected with T. cruzi. Mice were euthanized on day 30 after infection, and the liver and heart were subjected to histology and zymography to determine MMP-2 activities and plasma levels of IL-10, TNF, CCL2, and CCL5. T. cruzi-infected HFD animals had higher parasitemia, LDL and total cholesterol levels. Regardless of diet, plasma levels of all inflammatory mediators and cardiac MMP-2 activity were elevated in infected mice in contrast with the low plasma levels of leptin. HFD animals presented micro- and macrovesicular hepatic steatosis, while cardiac leukocyte infiltration was mainly detected in T. cruzi-infected mice. Our findings suggested that a HFD promotes higher circulating T. cruzi load and cardiac and liver immunopathogenesis in an experimental model using the VL-10 strain of the T. cruzi.