SAM Composition and Electrode Roughness Affect Performance of a DNA Biosensor for Antibiotic Resistance
SAM Composition and Electrode Roughness Affect Performance of a DNA Biosensor for Antibiotic Resistance
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DOI：
10.3390/bios9010022
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发表时间：
2019-02-07
期刊：
影响因子：
5.4
通讯作者：
Corrigan, Damion K.
中科院分区：
文献类型：
--
作者：
Butterworth, Adrian;Blues, Elizabeth;Corrigan, Damion K.
抗生素耐药性是全球感染性疾病治疗中日益关注的问题。快速识别样本中抗生素耐药性的护理点（Point-of-Care，简称PART）检测将允许立即进行靶向治疗，从而改善患者的治疗效果，并有助于维持当前抗生素库存的有效性。电化学分析提供了许多好处，但从台式测量系统转换为低成本便携式电极可能具有挑战性。利用电化学和物理技术，本研究探讨了不同的电极表面和生物识别元件，即自组装单层（SAM），如何影响生物传感器的性能，测量抗生素耐药性探针杂交到溶液中的靶基因序列。我们评估了几种市售的电极，这些电极可能适合于使用不同SAM层进行测试，并表明电极选择在整体生物传感器性能中也起着重要作用。
Antibiotic resistance is a growing concern in the treatment of infectious disease worldwide. Point-of-care (PoC) assays which rapidly identify antibiotic resistance in a sample will allow for immediate targeted therapy which improves patient outcomes and helps maintain the effectiveness of current antibiotic stockpiles. Electrochemical assays offer many benefits, but translation from a benchtop measurement system to low-cost portable electrodes can be challenging. Using electrochemical and physical techniques, this study examines how different electrode surfaces and bio-recognition elements, i.e. the self-assembled monolayer (SAM), affect the performance of a biosensor measuring the hybridisation of a probe for antibiotic resistance to a target gene sequence in solution. We evaluate several commercially available electrodes which could be suitable for PoC testing with different SAM layers and show that electrode selection also plays an important role in overall biosensor performance.