Molecular evolution of the TMS5 gene in rice (Oryza sativa L.)
Molecular evolution of the TMS5 gene in rice (Oryza sativa L.)
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水稻 TMS5 基因的分子进化 (Oryza sativa L.)
DOI：
10.1007/s10722-017-0533-1
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发表时间：
2017-06
影响因子：
2
通讯作者：
Chen Liangbi
中科院分区：
文献类型：
--
作者：
Tian Lianfu;Yuan Juan;Wang Xiaohui;Kang Tian;Hao Xiaohua;Li Dongping;Chen Liangbi
温敏核不育系已广泛应用于两系杂交水稻育种，但这些性状背后的TMS 5基因的进化特征仍不清楚。本研究选择TMS 5基因、启动子、3端基因组序列进行比较，结果表明该等位基因高度保守，具有不同的遗传来源。尽管存在多个非同义替换，但并未在所有水稻品种（包括栽培品种和野生品种）中检测到功能性核苷酸多态性。系统发育树分析表明，籼稻和粳稻是独立于野生祖先驯化的。此外，香稻在系统发育树中的多样性分布暗示香稻可能是从地理上孤立的群体中独立驯化的，这一发现与香稻起源于粳稻的假设不同。
Thermosensitive genic male-sterile lines have been extensively used in two-line hybrid rice breeding, yet the evolutionary characterization of the TMS5 gene underlying these traits remain unknown. In this study, we selected TMS5 gene, promoter, 3 terminal genomic region for sequence comparison and showed that this allele is highly conserved and has distinct genetic origins. Despite multiple nonsynonymous substitutions existed, the functional nucleotide polymorphism was not detected in all rice accessions, including cultivated varieties and wild accessions. Phylogenetic tree analysis showed that Indica and Japonica rice were domesticated independently from wild progenitors. Furthermore, the diverse distribution of Aromatic rice in the phylogenetic tree implied that Aromatic rice might be domesticated separately from geographically isolated populations and this finding differs from the assumption that Aromatic rice originated from the Japonica.