Peroxisome Fission is Associated with Reorganization of Specific Membrane Proteins
Peroxisome Fission is Associated with Reorganization of Specific Membrane Proteins
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DOI：
10.1111/j.1600-0854.2011.01198.x
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发表时间：
2011-07-01
期刊：
影响因子：
4.5
通讯作者：
Nagotu, Shirisha
中科院分区：
文献类型：
--
作者：
Cepinska, Malgorzata N.;Veenhuis, Marten;Nagotu, Shirisha
膜重塑是细胞器生物发生的一个重要方面。我们发现，在细胞器生物发生和增殖中发挥作用的不同过氧化物酶体膜蛋白（Pex8、Pex10、Pex14、Pex25 和 Pex11）在细胞器发育过程中受到时空行为的影响。通过对正常分裂过程中受阻的汉逊酵母 dnm1 细胞进行荧光显微镜分析，我们发现 Pex8、Pex10、Pex14 和 Pex25 的绿色荧光蛋白 (GFP) 融合体在出芽细胞中形成的细胞器延伸处显示出增强的荧光。相比之下，Pex11 荧光在母细胞器的延伸基部处富集。用作对照的 GFP 与转运蛋白 Pmp47 的融合蛋白在细胞器的任何特定区域均未显示出增强的荧光。删除 PEX11 或参与膜重塑的 Pex11 N 端结构域后，过氧化物酶体表面的特定过氧化物素浓度会丢失。在野生型细胞中观察到与 dnm1 细胞中类似的分布模式，其中 Pex8、Pex10、Pex14 和 Pex25，但不存在 Pex11，特别存在于迁移到芽的新形成的细胞器中。我们推测，过氧化物酶重组事件导致参与新生细胞器中过氧化物酶体生物合成的过氧化物酶水平提高。
Membrane remodeling is an important aspect in organelle biogenesis. We show that different peroxisome membrane proteins that play a role in organelle biogenesis and proliferation (Pex8, Pex10, Pex14, Pex25 and Pex11) are subject to spatiotemporal behavior during organelle development. Using fluorescence microscopy analysis of Hansenula polymorpha dnm1 cells that are blocked in the normal fission process, we show that green fluorescent protein (GFP) fusions of Pex8, Pex10, Pex14 and Pex25 show enhanced fluorescence at the organelle extensions that are formed in budding cells. In contrast, Pex11 fluorescence is enriched at the base of this extension on the mother organelle. A fusion protein of GFP with the transporter Pmp47, used as a control, did not show enhanced fluorescence at any specific region of the organelle. The concentration of specific peroxins at the peroxisome surface was lost upon deletion of PEX11 or the N-terminal domain of Pex11 that is involved in membrane remodeling. Comparable distribution patterns as in dnm1 cells were observed in wild-type cells where Pex8, Pex10, Pex14 and Pex25, but not Pex11, were especially present at newly formed organelles that migrated to the bud. We speculate that peroxin reorganization events result in enhanced levels of peroxins involved in peroxisome biogenesis in nascent organelles.