rDNA analyses as an aid to the taxonomy of species of Ganoderma
rDNA analyses as an aid to the taxonomy of species of Ganoderma
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DOI：
10.1017/s095375620000304x
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发表时间：
2000-09-01
影响因子：
--
通讯作者：
Wright, JE
中科院分区：
文献类型：
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作者：
Gottlieb, AM;Ferrer, E;Wright, JE
作为灵芝物种鉴定方法研究的一部分，本文描述了利用PCR偶联限制性消化、单链构象多态性（SSCP）和直接测序来测定南美灵芝标本rDNA多态性的方法。本研究的目的是确定形态上确定的群体是否可以通过分子标记进行区分，并检查ITS序列的变化。对7个形态学分类群的19株灵芝亚属和3个形态学分类群的30株灵芝亚属进行了研究。对21株分离株的ITS I区和ITS II区进行了序列分析。另外从EMBL/Genbank下载7个序列进行比较。总体而言，由限制模式划分的分组之间的一致性，ITS I和ITS II的SSCP是明显的。从ITS I、ITS II和组合数据集获得基因树并进行比较。在亚属水平上，分子和形态数据之间的一致性是明确的；然而，在具体层面上，这种关系是难以想象的。
As part of research to implement methods for the identification of Ganoderma species, this paper describes the use of PCR coupled to restriction digestions, single-strand conformational polymorphism (SSCP) and direct sequencing to assay rDNA polymorphism in South American collections of Ganoderma. The aim of the present study was to determine whether morphologically defined groups could be discriminated by molecular markers and to examine ITS sequence variation. Nineteen isolates of subgenus Ganoderma representing seven morphological taxa, and thirty isolates of subgenus Elfoingia representing three taxa, were studied. ITS I and IT II regions of twenty-one isolates were sequenced and were cladistically analyzed. Seven additional sequences were downloaded from EMBL/Genbank for comparison. In general, agreement between groupings delimited by restriction patterns, SSCP of ITS I and ITS II was evident. Gene trees derived from ITS I, ITS II and from a combined data set, were obtained and compared. Agreement between molecular and morphological data was clear at the subgeneric lever; however, at the specific level, this relationship was difficult to visualize.