An effective method for the discrimination of motional anisotropy and chemical exchange
An effective method for the discrimination of motional anisotropy and chemical exchange
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DOI：
10.1021/ja017461k
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发表时间：
2002-03-06
影响因子：
15
通讯作者：
Bracken, C
中科院分区：
文献类型：
--
作者：
Kneller, JM;Lu, M;Bracken, C
横向和纵向弛豫率之比 (R2/R1) 的分析是一种常用于估计总体相关时间和识别生物大分子化学交换的方法。然而，该分析未能区分化学交换和运动各向异性。我们描述了一种使用产品 R1R2 识别化学交换和运动各向异性的简单方法。在慢速翻滚状态中，R1R2 乘积产生与整体相关时间和运动各向异性无关的恒定值。该分析提供了一种简单的方法，可快速估计和分解 NMR 异核自旋弛豫数据中运动各向异性和化学交换的影响。我们通过在蛋白质E上收集的15N弛豫数据证明了该方法的实用性。大肠杆菌核糖核酸酶递三聚体E。共膜相关脂蛋白lpp。
Analysis of the ratio of transverse and longitudinal relaxation rates (R2/R1) is an approach commonly used for estimation of overall correlation time and identification of chemical exchange in biological macromolecules. However, this analysis fails to distinguish between chemical exchange and motional anisotropy. We describe a simple method for identifying chemical exchange and motional anisotropy using the product,R1R2. In the slow tumbling regime, theR1R2product results in a constant value that is independent of overall correlation time and motional anisotropy. This analysis provides a simple method for rapidly estimating and dissociating the effects of motional anisotropy and chemical exchange in NMR heteronuclear spin relaxation data. We demonstrate the utility of the method with15N relaxation data collected on the proteinsE. coli ribonuclease Hand the trimericE. colimembrane associated lipoprotein lpp.