Intracellular antibody neutralizes Listeria growth
Intracellular antibody neutralizes Listeria growth
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DOI：
10.1016/s1074-7613(01)00139-x
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发表时间：
2001-05-01
期刊：
影响因子：
32.4
通讯作者：
Unanue, ER
中科院分区：
文献类型：
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作者：
Edelson, BT;Unanue, ER
我们之前报道过，用抗李斯特菌溶血素O (LLO)（单核细胞增生李斯特菌的成孔毒素）的中和单抗治疗小鼠，提供了对这种细胞内细菌的抗性。我们评估了抗llo单抗是否会影响巨噬细胞对李斯特菌的处理，巨噬细胞是李斯特菌耐药性的必需细胞。在抗llo单抗存在下感染的巨噬细胞显示细胞内李斯特菌生长显著减少，同时llo依赖性李斯特菌从吞噬体向细胞质的传代受阻。抗LLO单抗不活化李斯特菌，而是在巨噬细胞内起作用来中和LLO。重要的是，抗llo单抗对李斯特菌生长的影响不依赖于Fc γ受体表达、IFN γ信号传导以及一氧化氮和超氧化物的产生。这些结果确定了巨噬细胞内细菌抗体控制的新机制。
We previously reported that treatment of mice with a neutralizing mAb against listeriolysin O (LLO), the pore-forming toxin of Listeria monocytogenes, provided resistance to this intracellular bacterium. We evaluated whether anti-LLO mAb would affect Listeria handling by macrophages, essential cells in Listeria resistance. Macrophages infected in the presence of anti-LLO mAb showed a marked reduction in intracellular Listeria growth, with a concomitant block in LLO-dependent Listeria passage from phagosome to cytosol. Anti-LLO mAb did not opsonize Listeria but, rather, acted within macrophages to neutralize LLO. Importantly, anti-LLO mAb effects on Listeria growth were independent of Fc gamma receptor expression, IFN gamma signaling, and production of nitric oxide and superoxide. These results identify a novel mechanism for antibody control of bacteria within macrophages.