Tracking single quantum dot and its spectrum in free solution with controllable thermal diffusion suppression
Tracking single quantum dot and its spectrum in free solution with controllable thermal diffusion suppression
复制标题
通过可控热扩散抑制跟踪自由溶液中的单个量子点及其光谱
DOI：
10.1016/j.ab.2008.03.017
复制
发表时间：
2008-06-15
影响因子：
2.9
通讯作者：
Yeung, Edward S.
中科院分区：
文献类型：
--
作者：
Li, Qun;Han, Rui;Yeung, Edward S.
半导体量子点（QD）的热运动被抑制在脱水琼脂糖改性的表面上。颗粒的扩散系数（D）可以通过用适当的琼脂糖浓度修饰表面来控制。当干燥的琼脂糖表面用8%琼脂糖溶液制成时，D值比理论值低100倍以上。这使得实时记录低粘度溶液中单个颗粒和单个分子的扩散过程成为可能。安装在电荷耦合器件前面的透射光栅将量子点荧光分成零级和一级光谱。因此，动态量子点的光谱在改性表面上被跟踪。动态量子点光谱图像跟踪是研究生理缓冲液中分子相互作用过程的一种很有前途的方法。(c)2008年爱思唯尔公司All rights reserved.
Thermal motions of semiconductor quantum dots (QDs) are suppressed on a dehydrated agarose-modified surface. The diffusion coefficients (D) of particles can be controlled by modifying the surface with an appropriate agarose concentration. The value of D is more than 100 times lower than the theoretical value when the dried agarose surface is made with an 8% agarose solution. This makes it possible to real-time record the diffusion process of single particles and single molecules in low-viscosity solution. A transmission grating installed in front of the charge-coupled device separates the QD fluorescence into the zeroth-order and first-order spectrum. Therefore, the spectrum of dynamic QDs is tracked on the modified surface. Tracking the dynamic QD spectral image is a promising method to explore the process of the molecular interactions in the physiological buffer. (c) 2008 Elsevier Inc. All rights reserved.