Immunohistochemical localization of acid-sensing ion channel 2 (ASIC2) in cutaneous Meissner and Pacinian corpuscles of Macaca fascicularis
Immunohistochemical localization of acid-sensing ion channel 2 (ASIC2) in cutaneous Meissner and Pacinian corpuscles of Macaca fascicularis
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DOI：
10.1016/j.neulet.2012.03.081
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发表时间：
2012-05-16
影响因子：
2.5
通讯作者：
Vega, J. A.
中科院分区：
文献类型：
--
作者：
Cabo, R.;Galvez, M. A.;Vega, J. A.
酸敏感离子通道2（ASIC 2）是变性蛋白/上皮钠通道超家族的成员，可能与机械感觉有关。ASIC 2的表达已在机械感觉神经元以及皮肤机械感受器的轴突和Schwann样细胞中检测到。在这些研究中，我们分析了ASIC 2的表达，在皮肤感觉小体的食蟹猴使用免疫组织化学和激光共聚焦扫描显微镜。ASIC 2在触觉小体和环层小体中均呈阳性反应。发现它与神经元特异性烯醇化酶和RT-97共定位，但不与S100蛋白共定位，表明ASIC 2表达仅限于供应机械感受器的轴突。这些结果首次证明了蛋白ASIC 2在猴子皮肤快速适应低阈值机械感受器中的存在，表明该离子通道在触觉中的作用。(c)2012爱思唯尔爱尔兰有限公司保留所有权利。
Acid-sensing ion channel 2 (ASIC2) is a member of the degenerin/epithelial sodium channel superfamily, presumably involved mechanosensation. Expression of ASIC2 has been detected in mechanosensory neurons as well as in both axons and Schwann-like cells of cutaneous mechanoreceptors. In these studies we analysed expression of ASIC2 in the cutaneous sensory corpuscles of Macaca fascicularis using immunohistochemistry and laser confocal-scanner microscopy. ASIC2 immunoreactivity was detected in both Meissner and Pacinian corpuscles. It was found to co-localize with neuron-specific enolase and RT-97, but not with S100 protein, demonstrating that ASIC2 expression is restricted to axons supplying mechanoreceptors. These results demonstrate for the first time the presence of the protein ASIC2 in cutaneous rapidly adapting low-threshold mechanoreceptors of monkey, suggesting a role of this ion channel in touch sense. (c) 2012 Elsevier Ireland Ltd. All rights reserved.