ESTABLISHMENT and CHARACTERISTICS OF A HUMAN PANCREATIC CANCER CELL LINE (HGC‐25)
ESTABLISHMENT and CHARACTERISTICS OF A HUMAN PANCREATIC CANCER CELL LINE (HGC‐25)
复制标题
人胰腺癌细胞系（HGC-25）的建立和特征
DOI：
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发表时间：
1977
期刊：
影响因子：
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通讯作者：
T. Kimoto
中科院分区：
文献类型：
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作者：
T. Akagi;T. Kimoto
从导管细胞来源的人胰腺癌的转移性腹水建立了漂浮细胞系（HGC-25）。该细胞系的特点是在悬浮液中生长，倍增时间为15.6小时，在软琼脂中的克隆效率高，众数为72。电子显微镜检查显示少数细胞中有终末条。未显示粘蛋白和免疫球蛋白的产生以及吞噬作用。细胞异种移植产生肿瘤，组织学上为未分化癌。上述特征证实HGC-25细胞系是人胰腺癌细胞系。
A floating cell line (HGC‐25) was established from the metastatic ascitic fluid of a human pancreatic adenocarcinoma of ductal cell origin. The cell line was characterized by the growth in suspension with a doubling time of 15.6 hr, a high cloning efficiency in soft agar and a modal chromosome number of 72. Electron microscopic examination revealed terminal bars in a small number of cells. Production of mucin and immunoglobulins and phagocytosis were not demonstrated. Heterotransplantation of the cells produced tumors, being undifferentiated carcinoma histologically. These characteristics mentioned above confirm that HGC‐25 cell line is a human pancreatic cancer ceU line.