Excited-state dynamics in the green fluorescent protein chromophore
Excited-state dynamics in the green fluorescent protein chromophore
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DOI：
10.1021/jp035816b
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发表时间：
2004-01-22
影响因子：
3.3
通讯作者：
Meech, SR
中科院分区：
文献类型：
--
作者：
Mandal, D;Tahara, T;Meech, SR
通过绿色荧光蛋白（GFP）在溶液中分离的发色团的超快荧光光谱研究了其对无辐射衰变的显着抑制。衰减数据通过荧光上转换测量，作为中性和阴离子形式发色团的溶剂和波长的函数。发现所有荧光衰减都可以通过两个指数衰减分量很好地描述。介质粘度的影响很小，表明促进无辐射衰变的分子内运动是一种体积守恒运动。观察到溶剂极性和氢键能力对衰减时间的微小影响。这两个衰减常数与发射波长无关，但它们的相对权重却不然。时间分辨荧光光谱表明斯托克斯位移在
The remarkable suppression of radiationless decay by the green fluorescent protein (GFP) is investigated through ultrafast fluorescence spectroscopy of its isolated chromophore in solution. Decay data are measured by fluorescence up-conversion as a function of solvent and wavelength for both neutral and anionic forms of the chromophore. All fluorescence decays are found to be well described by two exponentially decaying components. The effect of medium viscosity is slight, suggesting that the intramolecular motion promoting radiationless decay is a volume-conserving one. A minor effect of solvent polarity and H-bonding ability on the decay times is observed. The two decay constants are independent of emission wavelength, but their relative weights are not. Time-resolved fluorescence spectroscopy shows that the Stokes shift is complete in