Ethylene insensitive3-like2 (OsEIL2) confers stress sensitivity by regulating OsBURP16, the β subunit of polygalacturonase (PG1β-like) subfamily gene in rice
Ethylene insensitive3-like2 (OsEIL2) confers stress sensitivity by regulating OsBURP16, the β subunit of polygalacturonase (PG1β-like) subfamily gene in rice
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乙烯不敏感 3-like2 (OsEIL2) 通过调节 OsBURP16 赋予应激敏感性，OsBURP16 是水稻多聚半乳糖醛酸酶 (PG1β 样) 亚家族基因的 β 亚基
DOI：
10.1016/j.plantsci.2019.110353
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发表时间：
2020-03-01
期刊：
影响因子：
5.2
通讯作者：
Cai, Weiming
中科院分区：
文献类型：
--
作者：
Jin, Jing;Duan, Jianli;Cai, Weiming
转录因子EIN3(乙烯不敏感3)和EILS(EIN3-like)在植物发育和防御反应中发挥重要作用，但它们在这些过程中的作用机制尚不清楚。在这里，我们报道了水稻中一个类似EIN3的转录因子OsEIL2，它在非生物胁迫和叶片衰老中发挥了重要作用。OsEIL2是一种核定位蛋白，在C末端(氨基酸344-583)具有反式激活活性，可被氯化钠、聚乙二醇(PEG)、DLAK和脱落酸(ABA)诱导。与对照相比，过表达OsEIL2(OsEIL2-ox)的转基因植株对盐和干旱胁迫的耐受性降低。而高表达OsEIL2-RNA干扰(OsEIL2-RNAi)的转基因植株与对照相比，表现出更强的耐盐性和抗旱性。此外，过表达OsEIL2的转基因植株的幼苗表现出叶片发育延迟和加速黑暗诱导的衰老表型，而OsEIL2-RNAi植株表现出相反的表型。我们进一步发现，OsEIL2在OsBURP14和OsBURP16的上游起作用。OsBURP14和OsBURP16是多聚半乳糖醛酸酶亚家族的β亚基成员。OsBURP16过表达降低了水稻的果胶含量和细胞粘附性，提高了水稻对非生物胁迫的敏感性。OsEIL2直接与OsBURP14和OsBURP16的启动子结合并激活其转录水平。我们还发现，过表达OsEIL2通过增加多聚半乳糖醛酸酶(PG)的活性来降低果胶含量。综上所述，这些结果揭示了OsEIL2在非生物胁迫反应中的新机制。这些发现为研究植物对非生物胁迫的抗性提供了新的见解。
The transcription factors EIN3 (ETHYLENE-INSENSITIVE 3) and EILs (EIN3-Likes) play important roles in plant development and defense responses; however, their mechanism in these processes remain unclear. Here, we report that OsEIL2, an EIN3-like transcription factor from rice (Oryza sativa), plays important roles in abiotic stress and leaf senescence. OsEIL2 is a nuclear-localized protein with transactivation activity in the C-terminus (amino acids 344-583) and can be induced by NaCl, polyethylene glycol (PEG), dark, and abscisic acid (ABA) treatment. Transgenic plants of overexpressing OsEIL2 (OsEIL2-OX) show reduced tolerance to salt and drought stress compared with the controls. While the transgenic plants of overexpressing OsEIL2-RNA interference (OsEIL2-RNAi) exhibit enhanced tolerance to salt and drought stress compared with the controls. Moreover, seedlings of OsEIL2-overexpressing transgenic plants exhibit delayed leaf development and an accelerated dark-induced senescence phenotype, whereas OsEIL2-RNAi plants display the opposite phenotype. We further found that OsEIL2 functions upstream of OsBURP14 and OsBURP16. OsBURP14 and OsBURP16 are the members of the beta subunit of polygalacturonase subfamilies. OsBURP16 overexpression reduced pectin content and cell adhesion and increased abiotic stress sensitivity in rice. OsEIL2 binds directly to the promoter of OsBURP14 and OsBURP16 and activates their transcript levels. We also found that OsEIL2 overexpression decreased the pectin content by increasing polygalacturonase (PG) activity. Taken together, these results revealed a new mechanism of OsEIL2 in abiotic stress responses. These findings provide new insights into plant resistance to abiotic stress.