THE CAPSID PROTEIN OF SEMLIKI FOREST VIRUS HAS CLUSTERS OF BASIC AMINO-ACIDS AND PROLINES IN ITS AMINO-TERMINAL REGION
THE CAPSID PROTEIN OF SEMLIKI FOREST VIRUS HAS CLUSTERS OF BASIC AMINO-ACIDS AND PROLINES IN ITS AMINO-TERMINAL REGION
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DOI：
10.1073/pnas.77.11.6376
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发表时间：
1980-01-01
期刊：
影响因子：
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通讯作者：
DELIUS, H
中科院分区：
文献类型：
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作者：
GAROFF, H;FRISCHAUF, AM;DELIUS, H
衣壳(C)蛋白的氨基酸序列由C基因的核苷酸序列推导而来。病毒42S RNA基因组的这一部分被转录成双链cDNA。将该基因克隆到大肠杆菌.chi中。1776-pBR322宿主载体系统，用Maxam和Gilbert描述的技术测定碱基序列。C蛋白的氨基酸序列显示，前110个氨基酸中的碱性氨基酸和脯氨酸聚集在一起。
The amino acid sequence of the capsid (C) protein was deduced from the nucleotide sequence of the C gene. This part of the viral 42S RNA genome was transcribed into double-stranded cDNA. The cDNA was cloned in the Escherichia coli .chi. 1776-pBR322 host-vector system and then the base sequence was determined with the technique described by Maxam and Gilbert. The amino acid sequence of the C protein shows a clustering of basic amino acids and prolines within the first 110 amino acids.