Glucocorticoid receptors in rat kidney: the binding of tritiated-dexamethasone.
Glucocorticoid receptors in rat kidney: the binding of tritiated-dexamethasone.
复制标题
大鼠肾脏中的糖皮质激素受体：氚化地塞米松的结合。
作者：
J. Funder;D. Feldman;I. Edelman
糖皮质激素的作用被认为是通过与靶组织中的立体特异性细胞内受体蛋白结合而启动的。肾的各种功能受糖皮质激素的影响。在肾上腺切除的大鼠肾脏中，通过特异性结合地塞米松（DM）证实了高亲和力糖皮质激素受体。这些受体被命名为II型，并且可以基于对一系列生理和合成类固醇的不同亲和力而与高亲和力盐皮质激素受体（I型）和CBG样皮质酮受体（III型）区分开。通过Scatchard图分析，3 HDM-胞质受体复合物的平衡（解离）常数为5 × 10- 9 M（25 ℃），并且在胞质高速上清液（HSS）中结合位点的浓度为1.6 × 10-13摩尔/mg蛋白质。外皮层和髓质乳头中的受体浓度相似。II型受体对类固醇的亲和力...
The effects of glucocorticoid hormones are thought to be initiated by binding to stereospecific intracellular receptor proteins in target tissues. The kidney enjoys a variety of functions influenced by glucocorticoid hormones. High affinity glucocorticoid receptors have been demonstrated in adrenalectomized rat kidney by the specific binding of dexamethasone (DM). These receptors have been designated Type II, and can be distinguished from high affinity mineralocorticoid receptors (Type I) and the CBGlike corticosterone receptors (Type III) on the basis of differing affinities for a series of physiological and synthetic steroids. By Scatchard plot analysis, the equilibrium (dissociation) constant of the 3HDM–cytoplasmic receptor complex was 5 X 10-9M (25 C), and the concentration of binding sites 1.6 X 10-13 moles per mg protein in the cytoplasmic high speed supernatant (HSS). Similar concentrations of receptor were found in outer cortex and medulla—papilla. The affinity of the Type—II receptor for steroid...