DISCREPANCIES IN INTRACELLULAR BEHAVIOR OF 3 STRAINS OF TOBACCO MOSAIC VIRUS, 2 OF WHICH ARE SEROLOGICALLY INDISTINGUISHABLE
DISCREPANCIES IN INTRACELLULAR BEHAVIOR OF 3 STRAINS OF TOBACCO MOSAIC VIRUS, 2 OF WHICH ARE SEROLOGICALLY INDISTINGUISHABLE
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DOI：
10.1094/phyto-60-419
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发表时间：
1970-01-01
期刊：
影响因子：
3.2
通讯作者：
SHALLA, TA
中科院分区：
文献类型：
--
作者：
GRANETT, AL;SHALLA, TA
Attempts were made to distinguish two common strains of tobacco mosaic virus, U-1 (D) and U-1 (SB), and the U-5 strain by symptomatology, serology, and light and electron microscopy. In cells of Turkish tobacco (Nicotiana tabacum), the size, shape, and fine structure of the intracellular virus crystals formed by both U-1 (D) and U-1 (SB) were identical, but they were distinct from those formed by U-5. Also, the two common strains were indistinguishable from each other and different from U-5 on the basis of symptoms in various plants and in serological tests. The common strains appeared distinguishable only in differing tendencies to produce amorphous inclusions (X-bodies) and in the fre-quency with which the virus occurred in chloro-plasts. The U-1 (SB) strain was more effective than U-1 (D) or U-5 in causing the formation of X-bodies. The chloroplasts frequently contained virions of either U-1 (SB) or U-5, but only rarely U-1 (D). Phytopathology 60: 419-425.The sites of synthess of plant viruses in host cells have received considerable study in recent years (1, 5, 16, 18, 20). Earlier ideas that cellular organelles are involved in virus synthesis have lately received some support from electron microscope investigations (6, 7, 8, 23). A possible role in tobacco mosaic virus (TMV) synthesis has also been assigned to intracellular amorphous inclusions called X-bodies. Esau & Cronshaw (7) presented some findings in startling discrepancy with earlier investigations of TMV by electron microscopy (9, 14, 15, 17, 22, 24). Despite research by others, theirs were the first convincing micrographs showing particles of common TMV in chloroplasts and nuclei. They also described more fully the fine structure of X-bodies, and implied that both intraorganelle virions and X-bodies were frequent in thin sections of diseased tissue, contrary to previous observations.
