Somatic embryogenesis and shoot organogenesis from leaf explants of Primulina tabacum
Somatic embryogenesis and shoot organogenesis from leaf explants of Primulina tabacum
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报春花叶外植体的体细胞胚胎发生和芽器官发生
DOI：
10.1007/s11240-011-0087-4
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发表时间：
2012-05
期刊：
影响因子：
--
通讯作者：
Ma, Guohua
中科院分区：
文献类型：
--
作者：
Yang, Xingyu;Lu, Jinfeng;da Silva, Jaime A. Teixeira;Ma, Guohua
报春花是中国特有的珍稀濒危物种。建立有效的更新体系是保护和重新引入的必要条件。本研究以中国地区4种生态型植物的叶片为外植体，在含5μM噻二唑脲(TDZ)的Murashige和Skoog(MS)培养基上培养30d后，转入含5μM 6-苄基腺嘌呤(BA)的培养基中，观察到不定芽的发生。相反，叶片外植体在含5.0μM BA的培养基上培养30d，再转入含5.0μM TDZ的培养基中，可诱导体细胞胚胎发生。这表明，通过改变两种细胞分裂素在培养基中的添加顺序，可以简单地切换体细胞胚胎发生和芽器官发生。组织学研究表明，外植体在含有TDZ的培养液中孵育30天内即可诱导出胚性细胞。只有在诱导胚性细胞的情况下，TDZ才能促进体细胞胚胎发生，BA才能促进不定芽器官发生。比较不同生态型的外植体，湖南紫霞洞的叶片外植体在含有5μM TDZ和5 0μM BA的培养基上均能诱导出1~2个不定芽。而从其他3个生态生境采集的植物叶片，每个外植体可诱导50-70个体细胞胚胎/不定芽。此外，体细胞胚胎在不同的培养基上均能诱导二次体细胞胚胎发生和不定芽的发生。当移栽到生根基质上时，所有的再生芽都形成了不定根，经过驯化和移栽到1：1的沙子：蛭石混合物中，95%以上的试管苗成活。
Primulina tabacum is a rare and endangered species that is endemic to China. Establishing an efficient regeneration system is necessary for its conservation and reintroduction. In this study, when leaf explants collected from plants grown in four ecotypes in China are incubated on Murashige and Skoog (MS) medium containing 5.0 μM thidiazuron (TDZ) for 30 days, then transferred to medium containing 5.0 μM 6-benzyladenine (BA), adventitious shoots are then observed. Conversely, when leaf explants are incubated on medium containing 5.0 μM BA for 30 days, then transferred to medium containing 5.0 μM TDZ, somatic embryogenesis is induced. This indicates that somatic embryogenesis and shoot organogenesis could be switched simply by changing the order of two cytokinins supplemented in the culture medium. Histological investigation has revealed that embryogenic cells are induced within 30 days following incubation of explants in medium containing TDZ. Only if embryogenic cells were induced, TDZ could enhance somatic embryogenesis and BA could stimulate shoot organogenesis. When comparing explants from different ecotypes, leaf explants from Zixiadong in Hunan Province could induce low numbers (1–2) of either somatic embryos or adventitious shoots on medium containing either 5.0 μM TDZ or 5.0 μM BA, respectively. Whereas, leaf explants from plants collected from the other three ecological habitats could induce 50–70 somatic embryos/adventitious shoots per explant. Moreover, somatic embryos could induce secondary somatic embryogenesis and adventitious shoots on different media. All regenerated shoots developed adventitious roots when these are transferred to rooting medium, and over 95% of plantlets have survived following acclimatization and transfer to a potting mixture (1:1, sand:vermiculite).
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影响因子：
4.8
作者：
G. Ma;J. A. Teixeira da Silva;Guojiang Wu
通讯作者：
G. Ma;J. A. Teixeira da Silva;Guojiang Wu
影响因子：
64.8
作者：
通讯作者：
--
DOI：
--
发表时间：
2010
期刊：
--
影响因子：
--
作者：
Wu Wang;J. Ri;Nong Dong;Xu Wei
通讯作者：
Wu Wang;J. Ri;Nong Dong;Xu Wei
DOI：
10.1007/s11627-009-9257-z
发表时间：
2009-10
期刊：
In Vitro Cellular & Developmental Biology - Plant
影响因子：
--
作者：
Quan Xu;Zhe Hu;Chun-yuan Li;Xin-yu Wang;Chongying Wang
通讯作者：
Quan Xu;Zhe Hu;Chun-yuan Li;Xin-yu Wang;Chongying Wang
影响因子：
2.7
作者：
Liang, K. M.;Lin, Z. F.;Guan, L. L.
通讯作者：
Guan, L. L.