Melanocytes are potential immunocompetent cells: Evidence from recognition of immunological characteristics of cultured human melanocytes
Melanocytes are potential immunocompetent cells: Evidence from recognition of immunological characteristics of cultured human melanocytes
复制标题
DOI：
10.1034/j.1600-0749.2002.02065.x
复制
发表时间：
2002-12-01
期刊：
影响因子：
--
通讯作者：
Gu, JX
中科院分区：
文献类型：
--
作者：
Lu, Y;Zhu, WY;Gu, JX
树突性质、战略位置和其他有关黑素细胞免疫学特征的积累证据表明，它们不仅是专业的黑色素产生细胞，而且是免疫活性细胞。在这项研究中，我们证明培养的黑素细胞表达低水平的一些免疫学上重要的表面标记物，例如细胞间粘附分子-1和CD40。此外，我们首次报告黑素细胞的CD40表达可以通过干扰素-γ（IFN-γ）刺激上调。共培养 72 小时后，在 IFN-γ 浓度为 300 U/ml 时观察到 CD40 表达的最佳增强。在相同的治疗后还观察到最大黑素细胞驱动的 T 淋巴细胞增殖和白细胞介素 12 分泌，并被证明是 CD40 依赖性的。我们的数据进一步表明，CD40 连接后，黑素细胞上调其共刺激分子和粘附分子。除了之前关于黑素细胞的抗原处理和呈递能力的描述之外，我们因此假设了一个动态模型，其中黑素细胞交替充当异质抗原呈递细胞。由于细胞表面表达 CD40，在某些情况下，黑素细胞可能会通过 CD40 CD40L 相互作用直接接触并随后刺激 CD8+ 细胞毒性 T 淋巴细胞。
The dendritic nature, the strategic location, and other accumulated evidence about the immunologic characteristics of melanocytes suggest that they are not only professional melanin producing cells but are also immunocompetent cells. In this study, we demonstrated that cultured melanocytes express low levels of some immunologically important surface markers such as intercellular adhesion molecule-1 and CD40. Moreover, we report for the first time CD40 expression by melanocytes can be up-regulated by interferon-gamma ( IFN-gamma) stimulation. Optimal enhancement of CD40 expression was observed at an IFN-gamma concentration of 300 U/ml after a co-culture period of 72 h. Maximal melanocyte-driven T lymphocyte proliferation and interleukin-12 secretion were also observed following the same treatment and proved to be CD40-dependent. Our data further suggest that upon CD40 ligation, melanocytes up-regulate their co-stimulating and adhesion molecules. In addition to previous descriptions about the melanocyte's antigen processing and presenting capacity, we therefore hypothesize a dynamic model in which melanocytes alternatively work as heterogeneous antigen presenting cells. As a result of CD40 expression on the cell surface, melanocytes might contact and subsequently stimulate CD8+ cytotoxic T lymphocytes directly via CD40 CD40L interaction in some cases.