APL-1, A CAENORHABDITIS-ELEGANS GENE ENCODING A PROTEIN RELATED TO THE HUMAN BETA-AMYLOID PROTEIN-PRECURSOR
APL-1, A CAENORHABDITIS-ELEGANS GENE ENCODING A PROTEIN RELATED TO THE HUMAN BETA-AMYLOID PROTEIN-PRECURSOR
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DOI：
10.1073/pnas.90.24.12045
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发表时间：
1993-12-15
影响因子：
11.1
通讯作者：
LI, C
中科院分区：
文献类型：
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作者：
DAIGLE, I;LI, C
在阿尔茨海默病患者大脑中发现的老年斑的主要成分是β -淀粉样蛋白肽，它来源于更大的淀粉样蛋白前体蛋白（APP）。近年来，在不同的生物体中发现了许多APP及APP相关蛋白，构成了APP蛋白家族。我们从秀丽隐杆线虫中分离出几个编码app相关蛋白的cdna，并将相应的基因命名为apl-1。apl-1转录本经历两种形式的转录后修饰：反式剪接和选择性聚腺苷酸修饰。在体外翻译一个apl-1 cDNA产生一个大约预期大小的蛋白质。与果蝇、人类和小鼠的app相关蛋白类似，APL-1似乎不包含β -淀粉样肽。由于APP相关蛋白在进化过程中似乎是保守的，因此秀丽隐杆线虫的apl-1基因对于决定人类APP的正常功能应该是重要的。
The major component of senile plaques found in the brains of Alzheimer disease patients is the beta-amyloid peptide, which is derived from a larger amyloid precursor protein (APP). Recently, a number of APP and APP-related proteins have been identified in different organisms and constitute the family of APP proteins. We have isolated several cDNAs encoding an APP-related protein in the nematode Caenorhabditis elegans and have designated the corresponding gene as apl-1. The apl-1 transcripts undergo two forms of posttranscriptional modification: trans-splicing and alternative polyadenylylation. In vitro translation of an apl-1 cDNA results in a protein of approximately the expected size. Similar to the Drosophila, human, and mouse APP-related proteins, APL-1 does not appear to contain the beta-amyloid peptide. Because APP-related proteins seem to be conserved through evolution, the apl-1 gene from C. elegans should be important for determining the normal function of human APP.