EVALUATION OF SOYBEAN RFLP MARKER DIVERSITY IN ADAPTED GERM PLASM
EVALUATION OF SOYBEAN RFLP MARKER DIVERSITY IN ADAPTED GERM PLASM
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DOI：
10.1007/bf00222861
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发表时间：
1992-11-01
影响因子：
5.4
通讯作者：
FREESTONE, R
中科院分区：
文献类型：
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作者：
KEIM, P;BEAVIS, W;FREESTONE, R
大豆RFLP标记已经初步开发，并利用外来和适应基因型之间的广泛杂交进行遗传作图。我们已经筛选了38个大豆品系在128个RFLP标记位点，主要是为了表征种质结构，但也要评估的实用性RFLP标记在不适应人群中确定。在这些DNA探针中，70%检测到这组大豆品系的RFLP，平均多态性指数为0.30。这意味着5个标记基因座中只有1个在任何特定的适应大豆品系对之间是有信息的。与RFLP遗传距离（GD（R））的估计相关的方差进行了测定，所获得的值表明，使用超过65-90个标记位点的种质调查将增加很少的精度。GD（R）矩阵的聚类分析和主坐标分析表明，适应种质的多样性相对缺乏。在栽培品系中，几个适应美国南部成熟区的品系也作为一个单独的群体出现。将GD（R）数据与从系谱分析（GD（P））获得的遗传距离估计值进行比较。这两个措施相关的r=0.54的所有38行，但相关性增加到r=0.73时，只有适应行进行了分析。
Soybean RFLP markers have been primarily developed and genetically mapped using wide crosses between exotic and adapted genotypes. We have screened 38 soybean lines at 128 RFLP marker loci primarily to characterize germ plasm structure but also to evaluate the utility of RFLP markers identified in unadapted populations. Of these DNA probes 70% detected RFLPs in this set of soybean lines with an average polymorphism index of 0.30. This means that only 1 out of 5 marker loci was informative between any particular pair of adapted soybean lines. The variance associated with the estimation of RFLP genetic distance (GD(R)) was determined, and the value obtained suggested that the use of more than 65-90 marker loci for germ plasm surveys will add little precision. Cluster analysis and principal coordinate analysis of the GD(R) matrix revealed the relative lack of diversity in adapted germ plasm. Within the cultivated lines, several lines adapted to Southern US maturity zones also appeared as a separate group. GD(R) data was compared to the genetic distance estimates obtained from pedigree analysis (GD(P)). These two measures were correlated with r=0.54 for all 38 lines, but the correlation increased to r=0.73 when only adapted lines were analyzed.