The interplay of cell-cell and cell-substrate adhesion in collective cell migration
The interplay of cell-cell and cell-substrate adhesion in collective cell migration
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DOI：
10.1098/rsif.2014.0684
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发表时间：
2014-11-06
影响因子：
3.9
通讯作者：
Losert, Wolfgang
中科院分区：
文献类型：
--
作者：
Wang, Chenlu;Chowdhury, Sagar;Losert, Wolfgang
集体细胞迁移通常涉及显着的细胞-细胞和细胞-基质粘附以及接触细胞的高度协调运动。我们关注细胞-基质粘附和细胞-细胞粘附之间的相互作用。我们发现，细胞表面接触的丧失并不会显着改变快速迁移的变形虫细胞（盘基网柄菌）的突出和缩回的动态模式，但会显着改变它们粘附其他细胞的能力。对细胞形状动力学的分析表明，粘附在表面的细胞可以通过穿过细胞与细胞接触的突出波来协调其与邻近细胞的运动。然而，虽然如果细胞与表面分离则存在形状波，但它们不会将细胞与细胞耦合。此外，我们对肌动蛋白聚合的研究表明，细胞表面粘附的丧失会改变细胞与细胞接触处肌动蛋白的聚合。为了进一步研究细胞-细胞/细胞-基质相互作用，我们使用光学显微操作在受控位置形成细胞-基质接触。我们发现细胞形状动力学和细胞骨架活性都对细胞-基质接触的形成做出快速反应。
Collective cell migration often involves notable cell-cell and cell-substrate adhesions and highly coordinated motion of touching cells. We focus on the interplay between cell-substrate adhesion and cell-cell adhesion. We show that the loss of cell-surface contact does not significantly alter the dynamic pattern of protrusions and retractions of fast migrating amoeboid cells (Dictyostelium discoideum), but significantly changes their ability to adhere to other cells. Analysis of the dynamics of cell shapes reveals that cells that are adherent to a surface may coordinate their motion with neighbouring cells through protrusion waves that travel across cell-cell contacts. However, while shape waves exist if cells are detached from surfaces, they do not couple cell to cell. In addition, our investigation of actin polymerization indicates that loss of cell-surface adhesion changes actin polymerization at cell-cell contacts. To further investigate cell-cell/cell-substrate interactions, we used optical micromanipulation to form cell-substrate contact at controlled locations. We find that both cell-shape dynamics and cytoskeletal activity respond rapidly to the formation of cell-substrate contact.