Analysis of Mammalian Cell Cytoplasm with Electrophoresis in Nanometer Inner Diameter Capillaries.
Analysis of Mammalian Cell Cytoplasm with Electrophoresis in Nanometer Inner Diameter Capillaries.
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DOI：
10.1002/elan.200403240
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发表时间：
2005-07
期刊：
影响因子：
3
通讯作者：
L. A. Woods;Paula R Powell;T. L. Paxon;A. Ewing
中科院分区：
文献类型：
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作者：
L. A. Woods;Paula R Powell;T. L. Paxon;A. Ewing
在770纳米内径的毛细管中的毛细管电泳耦合到电化学检测，其具有与蚀刻毛细管匹配的蚀刻电极（蚀刻电化学检测），已经与超小采样一起用于注射来自完整的单个哺乳动物细胞的亚细胞样品。从大鼠嗜铬细胞瘤细胞中提取的细胞质样品的分离已经实现。仅对单个细胞总体积的8%进行了采样和分析。多巴胺已被确定和定量在这些PC12细胞使用这种技术。大鼠嗜铬细胞瘤细胞内多巴胺的平均水平为240 ± 60 μM。使用770纳米内径毛细管中的电泳和电化学检测来监测单细胞水平的细胞质神经递质，可以提供有关复杂细胞功能（例如神经递质储存和合成）的信息。
Capillary electrophoresis in 770 nanometer inner diameter capillaries coupled to electrochemical detection with an etched electrode matching an etched capillary (etched electrochemical detection) has been used with ultrasmall sampling to inject subcellular samples from intact single mammalian cells. Separations of cytoplasmic samples taken from rat pheochromocytoma cells have been achieved. As little as 8% of the total volume of a single cell has been sampled and analyzed. Dopamine has been identified and quantified in these PC12 cells using this technique. The average cytoplasmic level of dopamine in rat pheochromocytoma cells has been determined to be 240 ± 60 μM. The use of electrophoresis in 770 nanometer inner diameter capillaries with electrochemical detection to monitor cytoplasmic neurotransmitters at the single cell level can provide information about complex cellular functions such as neurotransmitter storage and synthesis.