Genetic variation in an apomictic grass, Heteropogon contortus, in the Hawaiian Islands

Genetic variation in an apomictic grass, Heteropogon contortus, in the Hawaiian Islands
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DOI:
10.1046/j.1365-294x.1999.00786.x
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发表时间:
1999-12-01
期刊:
影响因子:
4.9
通讯作者:
Daehler, CC
Daehler, CC
中科院分区:
生物学1区
文献类型:
--
作者:
Carino, DA;Daehler, CC

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随机扩增多态性DNA(RAPD)标记被用来评估夏威夷种群内和之间的遗传变异的无融合生殖草,Heterogopon contortus(皮利草)。从56个样本中,从6个群体的O'origin和夏威夷,55个独特的基因型检测到33个多态性标记。个体之间缺乏一致性可能表明这些种群中频繁的有性生殖。分子方差分析(AMOVA)显示,居群间变异显著(30.2%),居群内变异水平较高(68.1%)。聚类分析显示,大多数种群的聚类程度很高,但来自不同岛屿的种群没有聚类在一起。存在的人口分化,但缺乏岛屿之间的分化可能表明,一个contortus是一个早期波利尼西亚引进夏威夷群岛。
Random amplified polymorphic DNA (RAPD) markers were used to assess genetic variation within and among Hawaiian populations of an apomictic grass, Heterogopon contortus (pili grass). From among 56 individuals sampled from six populations on O'ahu and Hawai'i, 55 unique genotypes were detected using 33 polymorphic markers. This lack of uniformity among individuals may indicate frequent sexual reproduction in these populations. Analysis of molecular variance (AMOVA) revealed significant variation among populations (30.2%), but higher levels of variation within populations (68.1%). Cluster analysis revealed a high degree of clustering for most populations, but populations from different islands did not cluster together. The presence of among-population differentiation but lack of between-island differentiation may suggest that a contortus was an early Polynesian introduction to the Hawaiian islands.