Construction and characterization of a Bacteroides thetaiotaomicron recA mutant: Transfer of Bacteroides integrated conjugative elements is RecA independent
Construction and characterization of a Bacteroides thetaiotaomicron recA mutant: Transfer of Bacteroides integrated conjugative elements is RecA independent
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DOI：
10.1128/jb.179.20.6221-6227.1997
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发表时间：
1997-10-01
影响因子：
3.2
通讯作者：
Salyers, AA
中科院分区：
文献类型：
--
作者：
Cooper, AJ;Kalinowski, AP;Salyers, AA
我们报告的构建和分析的多形拟杆菌recA破坏突变体和调查是否RecA是需要切除和整合的拟杆菌移动的DNA元件。recA突变体是缺乏同源重组，比野生型菌株DNA损伤剂更敏感。recA突变体也比野生型对氧更敏感，表明DNA修复有助于B的耐氧性。太多了。许多拟杆菌临床分离株携带称为接合转座子的可自我传递的染色体元件。这些接合转座子也可以切除和移动在反式的一个家庭的未连接的整合元件称为非复制拟杆菌单位（NBU）。先前的研究结果提出了RecA在拟杆菌属接合转座子切除中起作用的可能性，但这一假设无法在拟杆菌属中得到验证。因为没有RecA缺陷拟杆菌菌株可用。我们在这里报告的切除和整合的拟杆菌接合转座子，以及NBU 1和Tn4351，不受RecA活性的情况下。
We report the construction and analysis of a Bacteroides thetaiotaomicron recA disruption mutant and an investigation of whether RecA is required for excision and integration of Bacteroides mobile DNA elements. The recA mutant was deficient in homologous recombination and was more sensitive than the wild-type strain to DNA-damaging agents. The recA mutant was also more sensitive to oxygen than the wild type, indicating that repair of DNA contributes to the aerotolerance of B. thetaiotaomicron. Many Bacteroides clinical isolates carry self-transmissible chromosomal elements known as conjugative transposons. These conjugative transposons can also excise and mobilize in trans a family of unlinked integrated elements called nonreplicating Bacteroides units (NBUs). The results of a previous study had raised the possibility that RecA plays a role in excision of Bacteroides conjugative transposons, but this hypothesis could not be tested in Bacteroides spp. because no RecA-deficient Bacteroides strain was available. We report here that the excision and integration of the Bacteroides conjugative transposons, as well as NBU1 and Tn4351, were unaffected by the absence of RecA activity.