Recording of microvascular dimensions with an image-splitter television microscope.
Recording of microvascular dimensions with an image-splitter television microscope.
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用图像分割电视显微镜记录微血管尺寸。
DOI：
10.1152/jappl.1966.21.1.299
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发表时间：
1966
影响因子：
3.3
通讯作者：
S. Báez
中科院分区：
文献类型：
--
作者：
S. Báez
贝兹西尔维奥用分像电视显微镜记录微血管尺寸。应用生理学杂志2 I（I）：2gg-30 '。1966.-一个系统已经开发了准确和快速的模拟记录微血管尺寸的变化。一个分像目镜剪切投影在电视显微镜屏幕上的血管图像。分光器测微计中的灵敏电位计的输出在测谎仪上准确记录图像剪切的量。该系统允许在离体组织和完整的血管床的微血管的机械和动态特性的评价。测微测量;毛细管;微血管尺寸记录;微循环评价微循环模式的微血管成分的单独活动所依据的机械性质需要：1）连续或快速顺序记录微血管事件和2）精确定量血管尺寸的变化。这种变化的连续记录最近已经完成，使用电视显微镜中视频信号的模拟计算（5）。然而，报告的标准误差为f 3.7~ 1时，该系统是校准对静态对象（玻璃毛细管），使其准确性低于经典的方法，显微测量与阶段校准的眼睛微米，特别是当处理较小的肌肉微动脉血管在哺乳动物。另一方面，通过将分像原理（2）应用于显微镜，可以提供显微图像测量的精度。该光学系统允许形成物体的双重图像，其中通过控制量剪切的图像通过单目目镜观看。这种测量可以提供“比光学系统的分辨能力好10倍或更多倍的精度”（3）。
BAEZ, SILVIO. Recording of microvascular dimensions with an image splitter television microscope. J. Appl. Physiol. 2 I (I): 2gg-30’. 1966.-A system has been developed for the accurate and rapid analogue recording of microvessel dimension changes. An image-splitter eyepiece shears the vascular image projected on the video screen of a television microscope. The output of a sensitive potentiometer incorporated in the micrometer of the splitter accurately registers on a polygraph the amount of image shearing. The system permits evaluation of the mechanical and dynamic characteristics of the microvessels in isolated tissues and the intact vascular bed. micrometric measurements; capillary; microvessel dimension recording; microcirculationEvaluation OF the mechanical properties underlying the separate activity of microvessel components of the microcirculatory pattern demands I) continuous or rapid sequential recording of the microvascular events and 2) accuracy in the quantitation of changes in vascular dimensions. Continuous recording of such changes was recently accomplished, using analogue computation from the video signal in the TV microscope (5). However, the reported standard error of f 3.7~ 1 when the system is calibrated against static objects (glass capillaries) renders it less accurate than the classic method of micrometric measurements with stage-calibrated ocular micrometers, particularly when dealing with smaller muscular microarterial vessels in mammals. On the other hand, precision in the measurement of a microscopic image can be provided by the application to the microscope of the image-splitting principle (2). This optical system allows for the formation of a double image of an object in which the image, sheared by a controlled amount, is viewed through the monocular eyepiece. Such measurements can provide an “accuracy better by a factor of IO or more than the resolving power of the optical system”(3).